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HDAC2 antibody

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX65924
Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65924
Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX65924
Anti-HDAC2 antibody used in Western Blot (WB). GTX65924
Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX65924
Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX65924

Cat. No. GTX65924

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all HDAC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human HDAC2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

Cellular Localization

Nucleus,Cytoplasm

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX65924

GTX65924 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX65924 HDAC2 antibody.
Dilution : 1:100

Anti-HDAC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65924

GTX65924 ICC/IF Image

ICC/IF analysis of A549 cells using GTX65924 HDAC2 antibody.
Blue : DAPI

Anti-HDAC2 antibody used in Immunoprecipitation (IP). GTX65924

GTX65924 IP Image

IP analysis of K562 cell lysate using GTX65924 HDAC2 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-HDAC2 antibody used in Western Blot (WB). GTX65924

GTX65924 WB Image

WB analysis of various sample lysates using GTX65924 HDAC2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX65924

GTX65924 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX65924 HDAC2 antibody.
Dilution : 1:100

Anti-HDAC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX65924

GTX65924 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX65924 HDAC2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399