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HDAC2 (phospho Ser394) antibody

Anti-HDAC2 (phospho Ser394) antibody used in Western Blot (WB). GTX50236
Anti-HDAC2 (phospho Ser394) antibody used in IHC (Paraffin sections) (IHC-P). GTX50236
Anti-HDAC2 (phospho Ser394) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50236
Anti-HDAC2 (phospho Ser394) antibody used in Western Blot (WB). GTX50236

Cat No. GTX50236

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from human HDAC2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

Cellular Localization

Nucleus,Cytoplasm

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-HDAC2 (phospho Ser394) antibody used in Western Blot (WB). GTX50236

GTX50236 WB Image

WB analysis of extracts from HepG2 cells treated with calf intestinal phosphatase (CIP) using GTX50236 HDAC2 (phospho Ser394) antibody.

Anti-HDAC2 (phospho Ser394) antibody used in IHC (Paraffin sections) (IHC-P). GTX50236

GTX50236 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50236 HDAC2 (phospho Ser394) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-HDAC2 (phospho Ser394) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50236

GTX50236 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50236 HDAC2 (phospho Ser394) antibody.

Anti-HDAC2 (phospho Ser394) antibody used in Western Blot (WB). GTX50236

GTX50236 WB Image

WB analysis of extracts from 293 cells untreated or treated with EGF using GTX50236 HDAC2 (phospho Ser394) antibody.

REFERENCE

There are currently no references for HDAC2 (phospho Ser394) antibody (GTX50236). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HDAC2 (phospho Ser394) antibody (GTX50236). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf