HDAC2 (phospho Ser394) antibody

Name: HDAC2 (phospho Ser394) antibody
Brand: GeneTex
SKU: GTX50236
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-HDAC2 (phospho Ser394) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50236

Anti-HDAC2 (phospho Ser394) antibody used in Western Blot (WB). GTX50236

Anti-HDAC2 (phospho Ser394) antibody used in IHC (Paraffin sections) (IHC-P). GTX50236

Cat. No. GTX50236

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399)

See all HDAC2 products

View product citations for antibody GTX50236 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200
IHC-P	1:50-1:100

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from human HDAC2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 2 , HD2 , KDAC2 , RPD3 , YAF1

Cellular Localization

Nucleus,Cytoplasm

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Gene ID: 3066 HDAC2
UniProt: Q92769 HDAC2

Research Area

DATA IMAGES

GTX50236 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50236 HDAC2 (phospho Ser394) antibody.

GTX50236 WB Image

WB analysis of extracts from 293 cells untreated or treated with EGF using GTX50236 HDAC2 (phospho Ser394) antibody.

GTX50236 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50236 HDAC2 (phospho Ser394) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

GTX50236 WB Image

WB analysis of extracts from HepG2 cells treated with calf intestinal phosphatase (CIP) using GTX50236 HDAC2 (phospho Ser394) antibody.

REFERENCE

Application Reference

Yen YT et al. Nat Commun 2021; 12 (1) : 7297 Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.

Application : IHC-P

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