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HDAC3 antibody [GT9007]

Anti-HDAC3 antibody [GT9007] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60366
Anti-HDAC3 antibody [GT9007] used in ChIP assay (ChIP assay). GTX60366

Cat. No. GTX60366

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT9007

Isotype

 IgG1

Application

 ICC/IF, ChIP assay

Reactivity

 Human
Package
50 μg ($399)
See all HDAC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:500
ChIP assay 1-5 μg
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2

Cellular Localization

Nucleus , Cytoplasm , Cytoplasm, cytosol

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC3 antibody [GT9007] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60366

GTX60366 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60366 HDAC3 antibody [GT9007].
Red : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-HDAC3 antibody [GT9007] used in ChIP assay (ChIP assay). GTX60366

GTX60366 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60366 HDAC3 antibody [GT9007]. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399