APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2
Cellular Localization
Nucleus,Cytoplasm,Cytoplasm, cytosol
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399](/templates/default/js/lightbox/images/loading.gif "Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399")
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GTX637399 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637399 IHC-P Image
HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.HDAC3 stained by HDAC3 antibody [HL1749] (GTX637399) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637399 IHC-P Image
HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.HDAC3 stained by HDAC3 antibody [HL1749] (GTX637399) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637399 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (GTX637399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for HDAC3 antibody [HL1749] (GTX637399). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HDAC3 antibody [HL1749] (GTX637399). Be the first to share your experience with this product.
![Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399](/upload/website/prouct_img/small/GTX637399/GTX637399_44837_20221021_WB_22102723_152.webp "Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399](/upload/website/prouct_img/small/GTX637399/GTX637399_T-44788_20221007_IHC-P_M_22110919_927.webp "Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399](/upload/website/prouct_img/small/GTX637399/GTX637399_T-44788_20221007_IHC-P_1_22110919_149.webp "Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399](/upload/website/prouct_img/small/GTX637399/GTX637399_44837_20221111_WB_M_R_22111518_444.webp "Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399](/upload/website/prouct_img/normal/GTX637399/GTX637399_44837_20221021_WB_22102723_152.webp "Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399](/upload/website/prouct_img/normal/GTX637399/GTX637399_T-44788_20221007_IHC-P_M_22110919_927.webp "Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399](/upload/website/prouct_img/normal/GTX637399/GTX637399_T-44788_20221007_IHC-P_1_22110919_149.webp "Anti-HDAC3 antibody [HL1749] used in IHC (Paraffin sections) (IHC-P). GTX637399")
![Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399](/upload/website/prouct_img/normal/GTX637399/GTX637399_44837_20221111_WB_M_R_22111518_444.webp "Anti-HDAC3 antibody [HL1749] used in Western Blot (WB). GTX637399")