APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| 1:100-1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , 293T , A431 , HeLa , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human HDAC3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10721050
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2
Cellular Localization
Nucleus
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113303 IP Image
HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody (GTX113303) 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX113303) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX113303 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113303 WB Image
HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted by 1:1000.
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GTX113303 ICC/IF Image
HDAC3 antibody detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody (GTX113303) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX113303 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113303 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113303 ChIP assay Image
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11651-6.
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GTX113303 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113303 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HDAC3(GTX113303) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113303 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500.
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GTX113303 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using HDAC3 antibody (GTX113303) at 1:300 dilution.
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GTX113303 IHC-Wm Image
Immunohistochemical analysis (whole mount) of zebrafish embryo, using HDAC3 antibody (GTX113303) at 1:200 dilution.
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GTX113303 WB IP Image
The data was published in the journal Nat Commun in 2017. PMID: 28916805
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REFERENCE
REVIEW
HDAC3 antibody
Cat. No. GTX113303
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 04-Jun-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
NB2a cells
Amount used :
5μg
Blocking :
10% skim milk, 25°C, 1Hr
Primary Antibody :
1:200, 25°C, 1Hr
