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HDAC4 antibody [4A3-H7-C6]

Anti-HDAC4 antibody [4A3-H7-C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49181
Anti-HDAC4 antibody [4A3-H7-C6] used in Western Blot (WB). GTX49181

Cat No. GTX49181

Host

Mouse

Clonality

Monoclonal

Clone Name

4A3-H7-C6

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human HDAC4 protein fragments expressed in E.coli

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Histone Deacetylase 4 , Aho3 , Bdmr , Ha6116 , Hd4 , Hdac-4 , Hdac-A , Hdaca , Hdac4

Cellular Localization

Nucleus. Cytoplasm

Background

Cytoplasm Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.

Database

Research Area

DATA IMAGES

Anti-HDAC4 antibody [4A3-H7-C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49181

GTX49181 ICC/IF Image

ICC/IF analysis of HeLa cells using HDAC4 antibody at a dilution of 1:150.

Anti-HDAC4 antibody [4A3-H7-C6] used in Western Blot (WB). GTX49181

GTX49181 WB Image

WB analysis of Jurkat, HeLa and K562 lysates using HDAC4 antibody at a dilution of 1:1,000.

REFERENCE

There are currently no references for HDAC4 antibody [4A3-H7-C6] (GTX49181). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HDAC4 antibody [4A3-H7-C6] (GTX49181). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf