GeneTex
  • Country / Location Selection

United States (US)

HDAC4 antibody [N3C1], Internal

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110231

Cat No. GTX110231

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 4 , AHO3 , BDMR , HA6116 , HD4 , HDAC-4 , HDAC-A , HDACA

Cellular Localization

Nucleus , Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110231

GTX110231 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4(GTX110231) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC4 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX110231

GTX110231 IP Image

HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal (GTX110231)
5 % SDS-PAGE
The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110231

GTX110231 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC4(GTX110231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

There are currently no reviews for HDAC4 antibody [N3C1], Internal (GTX110231). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169