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HDAC4 antibody [N3C1], Internal

KO/KD
Anti-HDAC4 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231
Anti-HDAC4 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX110231

Cat. No. GTX110231

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HDAC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

293T , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HDAC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10725876

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 4 , AHO3 , BDMR , HA6116 , HD4 , HDAC-4 , HDAC-A , HDACA

Cellular Localization

Nucleus , Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC4 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110231

GTX110231 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4(GTX110231) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110231

GTX110231 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC4(GTX110231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Western Blot (WB). GTX110231

GTX110231 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HDAC4 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX110231

GTX110231 IP Image

HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal (GTX110231)
5 % SDS-PAGE
The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Zhao B et al. Front Pharmacol 2022; 13 : 1010483 Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation.
Lai CY et al. Neuropharmacology 2018; 135 : 536-546 GluN2B/CaMKII mediates CFA-induced hyperalgesia via HDAC4-modified spinal COX2 transcription.
Application : WB, ICC/IF, IP
Reactivity : Rat
Sacilotto N et al. Genes Dev 2016; 30 : 2297-2309 MEF2 transcription factors are key regulators of sprouting angiogenesis.
Application : ICC/IF
Lin TB et al. J Pineal Res 2016; Melatonin relieves neuropathic allodynia through spinal MT2-enhanced PP2Ac and downstream HDAC4 shuttling-dependent epigenetic modification of hmgb1 transcription.
Application : WB
Reactivity : Rat
Lin TB et al. Anesthesiology 2015; 123 (1) : 199-212 Modulation of Nerve Injury-induced HDAC4 Cytoplasmic Retention Contributes to Neuropathic Pain in Rats.
Application : WB, IHC-Fr, IP
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169