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HDAC5 antibody

Anti-HDAC5 antibody used in Western Blot (WB). GTX50485
Anti-HDAC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX50485

Cat No. GTX50485

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.496~500 (T-Q-S-S-P) derived from human HDAC5.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 5 , HD5 , NY-CO-9

Cellular Localization

Nucleus,Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC5 antibody used in Western Blot (WB). GTX50485

GTX50485 WB Image

WB analysis of extracts from 293 and HepG2 cells using GTX50485 HDAC5 antibody.

Anti-HDAC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX50485

GTX50485 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50485 HDAC5 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for HDAC5 antibody (GTX50485). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HDAC5 antibody (GTX50485). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf