GeneTex
United States (US)

HDAC5 (phospho Ser498) antibody

GTX32287_20190131_WB_19013101_753.jpg
GTX32287_20190131_IHC-P_19013101_823.jpg
GTX32287_20190131_ICCIF_19013101_506.jpg

Cat No. GTX32287

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

122 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of HDAC5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone Deacetylase 5,Hd5,Ny-Co-9,Hdac5

Cellular Localization

Cytoplasm,Nucleus

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32287_20190131_WB_19013101_753.jpg

GTX32287 WB Image

WB analysis of LPS-treated HEK293T (A), TNFa-treated Raw264.7 (B), rat kidney (C) whole cell lysates using GTX32287 HDAC5 (phospho Ser498) antibody.

GTX32287_20190131_IHC-P_19013101_823.jpg

GTX32287 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32287 HDAC5 (phospho Ser498) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32287_20190131_ICCIF_19013101_506.jpg

GTX32287 ICC/IF Image

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32287 HDAC5 (phospho Ser498) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319