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HDAC6 antibody [4C5]

Overexpression
Anti-HDAC6 antibody [4C5] used in Western Blot (WB). GTX84377
Anti-HDAC6 antibody [4C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84377
Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377
Anti-HDAC6 antibody [4C5] used in Flow cytometry (FACS). GTX84377
Anti-HDAC6 antibody [4C5] used in Flow cytometry (FACS). GTX84377
Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377
Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377
Anti-HDAC6 antibody [4C5] used in Flow cytometry (FACS). GTX84377
Anti-HDAC6 antibody [4C5] used in Western Blot (WB). GTX84377
Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377

Cat. No. GTX84377

Reference 8

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($539)
See all HDAC6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10728413

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 6 , CPBHM , HD6 , JM21 , PPP1R90

Cellular Localization

Nucleus,Cytoplasm,Cell projection, dendrite

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

Database

Research Area

DATA IMAGES

Anti-HDAC6 antibody [4C5] used in Western Blot (WB). GTX84377

GTX84377 WB Image

Overexpression

WB analysis of HEK293T cells transfected with HDAC6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84377 HDAC6 antibody [4C5].
Loading : 5 ug per lane

Anti-HDAC6 antibody [4C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84377

GTX84377 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with HDAC6 plasmid using GTX84377 HDAC6 antibody [4C5].

Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377

GTX84377 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84377 HDAC6 antibody [4C5].
Dilution : 1:150

Anti-HDAC6 antibody [4C5] used in Flow cytometry (FACS). GTX84377

GTX84377 FACS Image

FACS analysis of HeLa cells using GTX84377 HDAC6 antibody [4C5].
Red : Primary antibody
Blue : Negative control antibody

Anti-HDAC6 antibody [4C5] used in Flow cytometry (FACS). GTX84377

GTX84377 FACS Image

FACS analysis of HEK293T cells transfected with either HDAC6 plasmid(Red) or empty vector control plasmid(Blue) using GTX84377 HDAC6 antibody [4C5].

Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377

GTX84377 IHC-P Image

IHC-P analysis of human liver tissue using GTX84377 HDAC6 antibody [4C5].
Dilution : 1:150

Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377

GTX84377 IHC-P Image

IHC-P analysis of human colon tissue using GTX84377 HDAC6 antibody [4C5].
Dilution : 1:150

Anti-HDAC6 antibody [4C5] used in Flow cytometry (FACS). GTX84377

GTX84377 FACS Image

FACS analysis of Jurkat cells using GTX84377 HDAC6 antibody [4C5].
Red : Primary antibody
Blue : Negative control antibody

Anti-HDAC6 antibody [4C5] used in Western Blot (WB). GTX84377

GTX84377 WB Image

WB analysis of various cell lines using GTX84377 HDAC6 antibody [4C5].
Loading : 35 ug per lane

Anti-HDAC6 antibody [4C5] used in IHC (Paraffin sections) (IHC-P). GTX84377

GTX84377 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84377 HDAC6 antibody [4C5].
Dilution : 1:150

REFERENCE

Application Reference

Lyu J et al. Mol Biol Cell 2023; 34 (8) : br11 The short-chain fatty acid acetate coordinates with CD30 to modulate T-cell survival.
Huang PY et al. Am J Cancer Res 2022; 12 (2) : 873-892 A novel HDAC11 inhibitor potentiates the tumoricidal effects of cordycepin against malignant peripheral nerve sheath tumor through the Hippo signaling pathway.
Application : WB
Reactivity : Human
Kim S et al. STAR Protoc 2021; 2 (3) : 100674 A protocol to visualize cytosolic aggresome-like bodies using confocal microscopy.
Application : ICC/IF
Reactivity : Human
Zhang L et al. Cell Mol Gastroenterol Hepatol 2020; (Epub) S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.
Application : ICC/IF
Reactivity : Mouse
Feng CW et al. Front Neurosci 2019; 13 : 769 Therapeutic Effect of Modulating TREM-1 via Anti-inflammation and Autophagy in Parkinson's Disease.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539