GeneTex
  • Country / Location Selection

United States (US)

HDAC6 antibody [N1], N-term

Anti-HDAC6 antibody [N1], N-term used in Western Blot (WB). GTX100722
Anti-HDAC6 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100722

Cat No. GTX100722

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

3xFlag-human HDAC6-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HDAC6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950485

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 6 , CPBHM , HD6 , JM21 , PPP1R90

Cellular Localization

Nucleus , Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HDAC6 antibody [N1], N-term used in Western Blot (WB). GTX100722

GTX100722 WB Image

HDAC6 antibody detects HDAC6 protein by western blot analysis. Non-transfected (-) and HDAC6-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC6 antibody (GTX100722) diluted by 1:5000.

Anti-HDAC6 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100722

GTX100722 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC6(GTX100722) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

HDAC6 antibody [N1], N-term Cat No. GTX100722

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 29-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : CT26 cells
Amount used : 30μg
Blocking : PBS, 5% BSA, and 0.1% Tween 20, 40 min, 25°C
Primary Antibody : 1:500, 4°C, > 12Hr
Figure Note : The observed M.W. is based on the publication: PMID: 29281743.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Antioxidants (Basel) in 2019. PMID: 31906147
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169