HDAC6 antibody [N1], N-term

Name: HDAC6 antibody [N1], N-term
Brand: GeneTex
SKU: GTX100722
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-HDAC6 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100722

Anti-HDAC6 antibody [N1], N-term used in Western Blot (WB). GTX100722

Cat. No. GTX100722

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX100722 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Positive Control

3xFlag-human HDAC6-transfected 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HDAC6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950485

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 6 , CPBHM , HD6 , JM21 , PPP1R90

Cellular Localization

Nucleus , Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq]

Database

Gene ID: 10013 HDAC6
UniProt: Q9UBN7 HDAC6

Research Area

DATA IMAGES

GTX100722 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC6(GTX100722) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX100722 WB Image

HDAC6 antibody detects HDAC6 protein by western blot analysis. Non-transfected (-) and HDAC6-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC6 antibody (GTX100722) diluted by 1:5000.

GTX100722 WB Image

The data was published in the journal Front Pharmacol in 2016.PMID: 27065869

REFERENCE

Application Reference

MC Lin et al. EMBO Rep 2024; Ago2/CAV1 interaction potentiates metastasis via controlling Ago2 localization and miRNA action

Hung YH et al. Am J Cancer Res 2022; 12 (2) : 713-728 Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.

Application : FACS

Reactivity : Human

Zhi-Hong Wen et al. Int J Mol Sci 2021; 22 (14) Chondroprotective Effects of a Histone Deacetylase Inhibitor, Panobinostat, on Pain Behavior and Cartilage Degradation in Anterior Cruciate Ligament Transection-Induced Experimental Osteoarthritic Rats.

Application : IHC-P

Reactivity : Rat

Lee JY et al. Front Pharmacol 2016; 7 : 81 Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.

Application : WB

Reactivity : Human

Lee KH et al. Sci Rep 2014; 4 : 6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

Application : WB

Reactivity : Human

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