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HDAC6 antibody

Anti-HDAC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02885
Anti-HDAC6 antibody used in Immunoprecipitation (IP). GTX02885
Anti-HDAC6 antibody used in Western Blot (WB). GTX02885
Anti-HDAC6 antibody used in IHC (Paraffin sections) (IHC-P). GTX02885

Cat. No. GTX02885

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HDAC6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone deacetylase 6 , CPBHM , HD6 , JM21 , PPP1R90

Cellular Localization

Nucleus , Cytoplasm , Cell projection, dendrite

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02885

GTX02885 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using using GTX02885 HDAC6 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-HDAC6 antibody used in Immunoprecipitation (IP). GTX02885

GTX02885 IP Image

IP analysis of HepG2 lysate using GTX02885 HDAC6 antibody.
Total extract : 100μg
IP antibody amount : 3μg
Dilution : 1:1000

Anti-HDAC6 antibody used in Western Blot (WB). GTX02885

GTX02885 WB Image

WB analysis of various samples using GTX02885 HDAC6 antibody.
Dilution : 1:1000
Loading : 25μg

Anti-HDAC6 antibody used in IHC (Paraffin sections) (IHC-P). GTX02885

GTX02885 IHC-P Image

IHC-P analysis of human liver tissue section using GTX02885 HDAC6 antibody.
Dilution : 1:400

REFERENCE

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REVIEW

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Package List Price ($)
$ 399