HDAC7 antibody immunoprecipitates HDAC7 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 μg of HDAC7 antibody (GTX114179) 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody (GTX114179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC7(GTX114179) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC7 antibody [C2C3], C-term (GTX114179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC7(GTX114179) antibody at 1:1000 dilution.