HDAC7 antibody [C2C3], C-term

Cat. No. GTX114179

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all HDAC7 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HDAC7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619437

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 7 , HD7 , HD7A , HDAC7A

Cellular Localization

Nucleus , Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 51564 HDAC7
UniProt: Q8WUI4 HDAC7

Research Area

DATA IMAGES

GTX114179 IP Image

HDAC7 antibody immunoprecipitates HDAC7 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC7 protein by 2 μg of HDAC7 antibody (GTX114179) 10% SDS-PAGE The immunoprecipitated HDAC7 protein was detected by HDAC7 antibody (GTX114179) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114179 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC7(GTX114179) antibody at 1:1000 dilution.

GTX114179 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC7 antibody [C2C3], C-term (GTX114179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 19484127

GTX114179 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC7(GTX114179) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty