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HDAC7 antibody

Anti-HDAC7 antibody used in Western Blot (WB). GTX12174
Anti-HDAC7 antibody used in Immunoprecipitation (IP). GTX12174

Cat. No. GTX12174

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all HDAC7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:50
IP 1.0-1.5 μg
IHC Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

NIH3T3, NRK

PROPERTIES

Form

Liquid

Buffer

0.01M PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding amino acids 436-452 of human HDAC7, conjugated to KLH.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 7 , HD7 , HD7A , HDAC7A

Cellular Localization

Nucleus,Cytoplasm

Background

HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Database

Research Area

DATA IMAGES

Anti-HDAC7 antibody used in Western Blot (WB). GTX12174

GTX12174 WB Image

WB analysis of HeLa cells using GTX12174 HDAC7 antibody at 1:500. Lane 1 : HeLa nuclear extract
Lane 2 : NIH-3T3

Anti-HDAC7 antibody used in Immunoprecipitation (IP). GTX12174

GTX12174 IP Image

IP analysis of HEK293T cells expressing DDDDK-tagged human HDAC7. The precipitated hHDAC7 protein was detected using anti-DDDDK Tag antibody.
Lane 1: IP from 180 μg of 293T cell extract (expressing DDDDK tagged human HDAC7) using 1.8 μg HDAC7 antibody(GTX12174)
Lane 2: Negative control (extract without antibody).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399