IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32364 HDAC8 (phospho Ser39) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

WB analysis of A549 (A), NIH3T3 (B), rat kidney (C) whole cell lysates using GTX32364 HDAC8 (phospho Ser39) antibody.

ICC/IF analysis of formalin-fixed A549 cells using GTX32364 HDAC8 (phospho Ser39) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes