GeneTex
United States (US)

HDAC8 (phospho Ser39) antibody

GTX32364_20190131_IHC-P_19013101_773.jpg
GTX32364_20190131_ICCIF_19013101_563.jpg
GTX32364_20190131_WB_19013101_830.jpg

Cat No. GTX32364

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

42 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of HDAC8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone Deacetylase 8,Cda07,Cdls5,Hd8,Hdacl1,Mrxs6,Rpd3,Wts,Hdac8

Cellular Localization

Cytoplasm,Nucleus

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX32364_20190131_IHC-P_19013101_773.jpg

GTX32364 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32364 HDAC8 (phospho Ser39) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32364_20190131_ICCIF_19013101_563.jpg

GTX32364 ICC/IF Image

ICC/IF analysis of formalin-fixed A549 cells using GTX32364 HDAC8 (phospho Ser39) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32364_20190131_WB_19013101_830.jpg

GTX32364 WB Image

WB analysis of A549 (A), NIH3T3 (B), rat kidney (C) whole cell lysates using GTX32364 HDAC8 (phospho Ser39) antibody.

Package List Price ($)
$ 319