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HE4 antibody [JF62-09]

Recombinant antibody
Anti-HE4 antibody [JF62-09] used in Western Blot (WB). GTX01063
Anti-HE4 antibody [JF62-09] used in IHC (Paraffin sections) (IHC-P). GTX01063
Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063
Anti-HE4 antibody [JF62-09] used in Flow cytometry (FACS). GTX01063
Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063

Cat No. GTX01063

Host

Rabbit

Clonality

Monoclonal

Clone Name

JF62-09

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Observed MW

23 kDa. The observed M.W. is based on the following publication. PMID: 31210752

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

,EDDM4,HE4,WAP fourdisulfide core domain 2,WAP5,WFDC2,dJ461P17.6

Cellular Localization

Secreted

Background

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-HE4 antibody [JF62-09] used in Western Blot (WB). GTX01063

GTX01063 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HE4 antibody [JF62-09] (GTX01063) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 31210752

Anti-HE4 antibody [JF62-09] used in IHC (Paraffin sections) (IHC-P). GTX01063

GTX01063 IHC-P Image

IHC-P analysis of mouse lung tissue section using GTX01063 HE4 antibody [JF62-09].

Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063

GTX01063 ICC/IF Image

ICC/IF analysis of PFA-fixed SW480 cells using GTX01063 HE4 antibody [JF62-09].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-HE4 antibody [JF62-09] used in Flow cytometry (FACS). GTX01063

GTX01063 FACS Image

FACS analysis of SW480 cells using GTX01063 HE4 antibody [JF62-09].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063

GTX01063 ICC/IF Image

ICC/IF analysis of PFA-fixed SKOV-3 cells using GTX01063 HE4 antibody [JF62-09].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for HE4 antibody [JF62-09] (GTX01063). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HE4 antibody [JF62-09] (GTX01063). Be the first to share your experience with this product.
Package List Price ($)
$ 369