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HE4 antibody [JF62-09]

Recombinant antibody
Anti-HE4 antibody [JF62-09] used in Western Blot (WB). GTX01063
Anti-HE4 antibody [JF62-09] used in IHC (Paraffin sections) (IHC-P). GTX01063
Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063
Anti-HE4 antibody [JF62-09] used in Flow cytometry (FACS). GTX01063
Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063

Cat No. GTX01063

Host

Rabbit

Clonality

Monoclonal

Clone Name

JF62-09

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Observed MW

23 kDa. The observed M.W. is based on the following publication. PMID: 31210752

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

EDDM4 , HE4 , WAP fourdisulfide core domain 2 , WAP5 , WFDC2 , dJ461P17.6

Cellular Localization

Secreted

Background

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HE4 antibody [JF62-09] used in Western Blot (WB). GTX01063

GTX01063 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HE4 antibody [JF62-09] (GTX01063) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 31210752

Anti-HE4 antibody [JF62-09] used in IHC (Paraffin sections) (IHC-P). GTX01063

GTX01063 IHC-P Image

IHC-P analysis of mouse lung tissue section using GTX01063 HE4 antibody [JF62-09].

Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063

GTX01063 ICC/IF Image

ICC/IF analysis of PFA-fixed SW480 cells using GTX01063 HE4 antibody [JF62-09].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-HE4 antibody [JF62-09] used in Flow cytometry (FACS). GTX01063

GTX01063 FACS Image

FACS analysis of SW480 cells using GTX01063 HE4 antibody [JF62-09].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-HE4 antibody [JF62-09] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01063

GTX01063 ICC/IF Image

ICC/IF analysis of PFA-fixed SKOV-3 cells using GTX01063 HE4 antibody [JF62-09].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for HE4 antibody [JF62-09] (GTX01063). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HE4 antibody [JF62-09] (GTX01063). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369