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HEBP1 antibody [N1C3]

Anti-HEBP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116121
Anti-HEBP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116121
Anti-HEBP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116121
Anti-HEBP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116121
Anti-HEBP1 antibody [N1C3] used in Western Blot (WB). GTX116121

Cat. No. GTX116121

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HEBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HEBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633179

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heme binding protein 1 , HBP , HEBP

Cellular Localization

Cytoplasm

Background

The full-length protein encoded by this gene is an intracellular tetrapyrrole-binding protein. This protein includes a natural chemoattractant peptide of 21 amino acids at the N-terminus, which is a natural ligand for formyl peptide receptor-like receptor 2 (FPRL2) and promotes calcium mobilization and chemotaxis in monocytes and dendritic cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HEBP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116121

GTX116121 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HEBP1(GTX116121) antibody at 1:400 dilution.

Anti-HEBP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116121

GTX116121 IHC-P Image

HEBP1 antibody [N1C3] detects HEBP1 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HEBP1 antibody [N1C3] (GTX116121) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HEBP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116121

GTX116121 IHC-P Image

HEBP1 antibody [N1C3] detects HEBP1 protein at cytoplasm in mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
HEBP1 antibody [N1C3] (GTX116121) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HEBP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116121

GTX116121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using HEBP1(GTX116121) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HEBP1 antibody [N1C3] used in Western Blot (WB). GTX116121

GTX116121 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX116121 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169