Summary
HEK293 cells from ATCC (Cat# CRL-1573) were grown to 85% confluency, harvested, homogenized in modified RIPA buffer and cleared by centrifugation. Modified RIPA buffer. Protein concentration of the cell lysate was determined, and the lysate was boiled for 5 min in SDS sample buffer.
Form
Liquid
Buffer
1x SDS sample buffer, 5% β-mercaptoethanol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Whole Cell Lysate
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.