GeneTex
United States (US)

HELIOS antibody [N3C3]

Orthogonal
GTX115629_40555_IHC_18121410_885.jpg
GTX115629_40555_WB_Fraction_18121410_691.jpg

Cat No. GTX115629

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Jurkat , Jurkat NE

Predict Reactivity

Mouse, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HELIOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HELIOS antibody, MGC34330 antibody, ZNF1A2 antibody, ZNFN1A2 antibody, IKZF2 antibody, zinc finger DNA binding protein Helios antibody, ikaros family zinc finger protein 2 antibody, "zinc finger protein, subfamily 1A, 2 (Helios) antibody", zinc finger protein Helios antibody, IKAROS family zinc finger 2 (Helios) antibody

Cellular Localization

Nucleus

Background

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]
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Database

DATA IMAGES
GTX115629_40555_IHC_18121410_885.jpg

GTX115629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS(GTX115629) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115629_40555_WB_Fraction_18121410_691.jpg

GTX115629 WB Image

Orthogonal

HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody (GTX115629) diluted by 1:500 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169