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HELIOS antibody [N3C3]

Orthogonal
Anti-HELIOS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115629
Anti-HELIOS antibody [N3C3] used in Western Blot (WB). GTX115629

Cat. No. GTX115629

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HELIOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Jurkat , Jurkat NE

Predict Reactivity

Mouse, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HELIOS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167260

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IKAROS family zinc finger 2 , ANF1A2 , HELIOS , ZNF1A2 , ZNFN1A2

Cellular Localization

Nucleus

Background

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HELIOS antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115629

GTX115629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS(GTX115629) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HELIOS antibody [N3C3] used in Western Blot (WB). GTX115629

GTX115629 WB Image

Orthogonal

HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody (GTX115629) diluted by 1:500 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Moysi E et al. Front Immunol 2021; 12 : 683396 In Situ Characterization of Human Lymphoid Tissue Immune Cells by Multispectral Confocal Imaging and Quantitative Image Analysis; Implications for HIV Reservoir Characterization.
Amodio D et al. J Immunol 2017; (Epub) Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination.
Application : IHC
Reactivity : Human
Muto S et al. Int J Oncol 2015; 47 (6) : 2082-90 Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169