APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
EL4 Cell Lysate(GTX27183) , Human Spleen Tissue Slide
Product Note
At least two isoforms are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other IKZF proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
HELIOS antibody was raised against a 19 amino acid peptide near the amino terminus of human HELIOS.The immunogen is located within amino acids 50 - 100 of HELIOS.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
IKAROS family zinc finger 2 , ANF1A2 , HELIOS , ZNF1A2 , ZNFN1A2
Cellular Localization
Nucleus
Background
This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX31612 WB Image
WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31612 HELIOS antibody.
Working concentration : 1 μg/ml
|
|
|
GTX31612 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31612 HELIOS antibody.
Working concentration : 20 μg/ml
|
|
|
GTX31612 IHC-P Image
IHC-P analysis of human spleen tissue using GTX31612 HELIOS antibody.
Working concentration : 5 μg/ml
|
REFERENCE
There are currently no references for HELIOS antibody (GTX31612). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HELIOS antibody (GTX31612). Be the first to share your experience with this product.
