GeneTex
United States (US)

HELIOS antibody

GTX31612_1272_IHC-P_20180221_18121410_201.jpg
GTX31612_1273_IHC-P_20180221_18121410_286.jpg
GTX31612_2797_WB_20180221_18121410_971.jpg

Cat No. GTX31612

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

EL4 Cell Lysate(GTX27183) , Human Spleen Tissue Slide

Specificity/Sensitivity

At least two isoforms are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other IKZF proteins.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HELIOS antibody was raised against a 19 amino acid peptide near the amino terminus of human HELIOS.The immunogen is located within amino acids 50 - 100 of HELIOS.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ikaros Family Zinc Finger 2,Anf1A2,Helios,Znf1A2,Znfn1A2,Ikzf2

Background

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX31612_1272_IHC-P_20180221_18121410_201.jpg

GTX31612 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31612 HELIOS antibody.
Working concentration : 5 μg/ml

GTX31612_1273_IHC-P_20180221_18121410_286.jpg

GTX31612 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31612 HELIOS antibody.
Working concentration : 20 μg/ml

GTX31612_2797_WB_20180221_18121410_971.jpg

GTX31612 WB Image

WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31612 HELIOS antibody.
Working concentration : 1 μg/ml

Package List Price ($)
$ 329