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HELLS antibody

KO/KD
Anti-HELLS antibody used in Western Blot (WB). GTX54157
Anti-HELLS antibody used in IHC (Paraffin sections) (IHC-P). GTX54157
Anti-HELLS antibody used in Western Blot (WB). GTX54157
Anti-HELLS antibody used in Immunoprecipitation (IP). GTX54157

Cat. No. GTX54157

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HELLS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

helicase, lymphoid specific , ICF4 , LSH , Nbla10143 , PASG , SMARCA6

Cellular Localization

Nucleus

Background

This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-HELLS antibody used in Western Blot (WB). GTX54157

GTX54157 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX54157 HELLS antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HELLS antibody used in IHC (Paraffin sections) (IHC-P). GTX54157

GTX54157 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX54157 HELLS antibody.
Dilution : 1:100

Anti-HELLS antibody used in Western Blot (WB). GTX54157

GTX54157 WB Image

WB analysis of various sample lysates using GTX54157 HELLS antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HELLS antibody used in Immunoprecipitation (IP). GTX54157

GTX54157 IP Image

IP analysis of 293T cell lysate using GTX54157 HELLS antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

REFERENCE

Application Reference

Stefanie Schuller et al. Cancers (Basel) 2022; 14 (2) HELLS Is Negatively Regulated by Wild-Type P53 in Liver Cancer by a Mechanism Involving P21 and FOXM1.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399