GeneTex
United States (US)

HENMT1 antibody

Anti-HENMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107190
Anti-HENMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107190
Anti-HENMT1 antibody used in Western Blot (WB). GTX107190

Cat No. GTX107190

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HENMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hen Methyltransferase 1,C1Orf59,Hen1,Henmt1

Database

DATA IMAGES
Anti-HENMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107190

GTX107190 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LOC113802(GTX107190) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-HENMT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107190

GTX107190 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LOC113802(GTX107190) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HENMT1 antibody used in Western Blot (WB). GTX107190

GTX107190 WB Image

Sample
A: 30μg of H1299 whole cell lysate
B: 30μg of HeLa S3 whole cell lysate (GTX14654)
C: 30μg of Hep G2 whole cell lysate (GTX27900)
7.5% SDS PAGE
GTX107190 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169