GeneTex

  • Country / Location Selection

United States (US)

HERC5 antibody, Internal

KO/KD
Anti-HERC5 antibody, Internal used in Western Blot (WB). GTX46920
Anti-HERC5 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX46920
Anti-HERC5 antibody, Internal used in Western Blot (WB). GTX46920

Cat. No. GTX46920

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($409)
See all HERC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

HepG2 , Transfected HEK293

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human HERC5

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HECT and RLD domain containing E3 ubiquitin protein ligase 5 , CEB1 , CEBP1

Background

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HERC5 antibody, Internal used in Western Blot (WB). GTX46920

GTX46920 WB Image

WB analysis of HepG2 cells using GTX46920 HERC5 antibody at 0.2-1μg/ml.

Anti-HERC5 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX46920

GTX46920 IHC-P Image

IHC-P analysis of human pineal tissue using GTX46920 HERC5 antibody at 1:100. Left to right:DAPI, HERC5, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

Anti-HERC5 antibody, Internal used in Western Blot (WB). GTX46920

GTX46920 WB Image

KO/KD

WB analysis of HEK293 cells over-expressing HERC5 protein using GTX46920 HERC5 antibody at 1:1000.
Lane 1 : HEK293 cells overexpressing flag tagged HERC5 protein
Lane 2 : HEK293 cells overexpressing flag tagged HERC5 protein + HERC5 shRNA

REFERENCE

There are currently no references for HERC5 antibody, Internal (GTX46920). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for HERC5 antibody, Internal (GTX46920). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409