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HES1 antibody [4H1]

Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375
Anti-HES1 antibody [4H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84375
Anti-HES1 antibody [4H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84375
Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375
Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375
Anti-HES1 antibody [4H1] used in Western Blot (WB). GTX84375
Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375
Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375

Cat. No. GTX84375

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all HES1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hes family bHLH transcription factor 1 , HES-1 , HHL , HRY , bHLHb39

Cellular Localization

Nucleus

Background

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.

Database

Research Area

DATA IMAGES

Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375

GTX84375 IHC-P Image

IHC-P analysis of breast tissue using GTX84375 HES1 antibody [4H1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-HES1 antibody [4H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84375

GTX84375 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with HES1 plasmid using GTX84375 HES1 antibody [4H1].

Anti-HES1 antibody [4H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84375

GTX84375 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84375 HES1 antibody [4H1].

Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375

GTX84375 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84375 HES1 antibody [4H1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375

GTX84375 IHC-P Image

IHC-P analysis of liver tissue using GTX84375 HES1 antibody [4H1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-HES1 antibody [4H1] used in Western Blot (WB). GTX84375

GTX84375 WB Image

WB analysis of HEK293T cells transfected with HES1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84375 HES1 antibody [4H1].
Loading : 5 ug per lane

Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375

GTX84375 IHC-P Image

IHC-P analysis of kidney tissue using GTX84375 HES1 antibody [4H1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-HES1 antibody [4H1] used in IHC (Paraffin sections) (IHC-P). GTX84375

GTX84375 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84375 HES1 antibody [4H1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519