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HES1 antibody [HL2198]

Recombinant antibody Orthogonal
Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202
Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202
Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202
Anti-HES1 antibody [HL2198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638202

Cat. No. GTX638202

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2198

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HES1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human HES1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hes family bHLH transcription factor 1 , HES-1 , HHL , HRY , bHLHb39

Cellular Localization

Nucleus

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202

GTX638202 WB Image

Orthogonal

MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202

GTX638202 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HES1 antibody [HL2198] used in Western Blot (WB). GTX638202

GTX638202 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (GTX638202) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HES1 antibody [HL2198] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638202

GTX638202 ICC/IF Image

HES1 antibody [HL2198] detects HES1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HES1 stained by HES1 antibody [HL2198] (GTX638202) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations