GeneTex
United States (US)

HES1 antibody [N1], N-term

GTX108356_39946_WB_R_19011507_311.jpg
GTX108356_39946_20150410_IFA_19011507_582.jpg
GTX108356_39946_IP_19011507_720.jpg
GTX108356_WB_19011507_167.jpg
GTX108356_39946_WB_M_kidney_19011507_484.jpg

Cat No. GTX108356

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse kidney , PC-12

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HES1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ20408 antibody, HES-1 antibody, HHL antibody, HRY antibody, bHLHb39 antibody, HES1 antibody, hairy-like protein antibody, hairy homolog antibody, class B basic helix-loop-helix protein 39 antibody, transcription factor HES-1 antibody, "hairy and enhancer of split 1, (Drosophila) antibody"

Cellular Localization

Nucleus

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX108356_39946_WB_R_19011507_311.jpg

GTX108356 WB Image

HES1 antibody [N1], N-term detects HES1 protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
12% SDS-PAGE
HES1 antibody [N1], N-term (GTX108356) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108356_39946_20150410_IFA_19011507_582.jpg

GTX108356 ICC/IF Image

HES1 antibody [N1], N-term detects HES1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HES1 protein stained by HES1 antibody [N1], N-term (GTX108356) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX108356_39946_IP_19011507_720.jpg

GTX108356 IP Image

HES1 antibody immunoprecipitates HES1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 μg of HES1 antibody (GTX108356) 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody (GTX108356) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX108356_WB_19011507_167.jpg

GTX108356 WB Image

Sample(30 µg whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX108356 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108356_39946_WB_M_kidney_19011507_484.jpg

GTX108356 WB Image

HES1 antibody [N1], N-term detects HES1 protein by western blot analysis.
A.50 µg mouse kidney extract
12% SDS-PAGE
HES1 antibody [N1], N-term (GTX108356) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf