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HEY1 antibody

Orthogonal
Anti-HEY1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118007
Anti-HEY1 antibody used in Western Blot (WB). GTX118007
Anti-HEY1 antibody used in Western Blot (WB). GTX118007

Cat. No. GTX118007

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HEY1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

DDDDK-tagged HEY1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HEY1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168085

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hes related family bHLH transcription factor with YRPW motif 1 , BHLHb31 , CHF2 , HERP2 , HESR1 , HRT-1 , NERP2 , OAF1 , hHRT1

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HEY1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118007

GTX118007 ICC/IF Image

HEY1 antibody detects HEY1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HEY1 protein stained by HEY1 antibody (GTX118007) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-HEY1 antibody used in Western Blot (WB). GTX118007

GTX118007 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody (GTX118007) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HEY1 antibody used in Western Blot (WB). GTX118007

GTX118007 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2020.PMID: 32366833

REFERENCE

Application Reference

Wei ST et al. Cell Death Dis 2020; 11 (5) : 307 Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling.
Application : WB
Ma L et al. Biochem Biophys Res Commun 2014; (Epub) Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169