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HGF antibody

Anti-HGF antibody used in Western Blot (WB). GTX32651
Anti-HGF antibody used in IHC (Paraffin sections) (IHC-P). GTX32651
Anti-HGF antibody used in IHC (Paraffin sections) (IHC-P). GTX32651

Cat. No. GTX32651

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-285 of human HGF (NP_001010933.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatocyte growth factor , DFNB39 , F-TCF , HGFB , HPTA , SF

Background

This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-HGF antibody used in Western Blot (WB). GTX32651

GTX32651 WB Image

WB analysis of HepG2 cell lysate using GTX32651 HGF antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-HGF antibody used in IHC (Paraffin sections) (IHC-P). GTX32651

GTX32651 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32651 HGF antibody.
Dilution : 1:100

Anti-HGF antibody used in IHC (Paraffin sections) (IHC-P). GTX32651

GTX32651 IHC-P Image

IHC-P analysis of mouse spinal cord tissue using GTX32651 HGF antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399