GeneTex
United States (US)

HGS antibody [C2C3], C-term

GTX101718_WB_19011506_962.jpg
GTX101718_39876_IFA_19011506_301.jpg
GTX101718_39876_WB_M_brain_19011506_268.jpg
GTX101718_39876_WB_R_brain_19011506_946.jpg
GTX101718_39876_IHC_19011506_364.jpg

Cat No. GTX101718

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain , rat brain

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HGS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate,Hrs,Hgs

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein; Cytoplasmic side , Endosome , multivesicular body membrane; Peripheral membrane protein

Background

The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101718_WB_19011506_962.jpg

GTX101718 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
B: HeLa
7.5% SDS PAGE
GTX101718 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101718_39876_IFA_19011506_301.jpg

GTX101718 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HGS(GTX101718) antibody (green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:500.

GTX101718_39876_WB_M_brain_19011506_268.jpg

GTX101718 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX101718 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101718_39876_WB_R_brain_19011506_946.jpg

GTX101718 WB Image

HGS antibody [C2C3], C-term detects HGS protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
HGS antibody [C2C3], C-term (GTX101718) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101718_39876_IHC_19011506_364.jpg

GTX101718 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HGS(GTX101718) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169