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HHIP antibody [HL1737]

Recombinant antibody Orthogonal
Anti-HHIP antibody [HL1737] used in Western Blot (WB). GTX637385
Anti-HHIP antibody [HL1737] used in IHC (Paraffin sections) (IHC-P). GTX637385
Anti-HHIP antibody [HL1737] used in IHC (Paraffin sections) (IHC-P). GTX637385

Cat. No. GTX637385

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1737

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HHIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HHIP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hedgehog interacting protein , HIP

Cellular Localization

Cell membrane,Secreted

Background

This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-HHIP antibody [HL1737] used in Western Blot (WB). GTX637385

GTX637385 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1737] (GTX637385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HHIP antibody [HL1737] used in IHC (Paraffin sections) (IHC-P). GTX637385

GTX637385 IHC-P Image

HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HHIP stained by HHIP antibody [HL1737] (GTX637385) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HHIP antibody [HL1737] used in IHC (Paraffin sections) (IHC-P). GTX637385

GTX637385 IHC-P Image

HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HHIP stained by HHIP antibody [HL1737] (GTX637385) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations