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HHIP antibody [N3C2], Internal

Orthogonal Overexpression
Anti-HHIP antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112584
Anti-HHIP antibody [N3C2], Internal used in Western Blot (WB). GTX112584
Anti-HHIP antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112584
Anti-HHIP antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112584
Anti-HHIP antibody [N3C2], Internal used in Western Blot (WB). GTX112584
Anti-HHIP antibody [N3C2], Internal used in Western Blot (WB). GTX112584

Cat. No. GTX112584

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Chicken
Package
100 μl ($399),
25 μl ($169)
See all HHIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HHIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037165

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hedgehog interacting protein , HIP

Cellular Localization

Cell membrane; Peripheral membrane protein , Secreted , Isoform 2: Cytoplasm

Background

This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HHIP antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112584

GTX112584 IHC-P Image

HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HHIP antibody [N3C2], Internal (GTX112584) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HHIP antibody [N3C2], Internal used in Western Blot (WB). GTX112584

GTX112584 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HHIP antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112584

GTX112584 IHC-P Image

HHIP antibody [N3C2], Internal detects HHIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
HHIP stained by HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-HHIP antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112584

GTX112584 ICC/IF Image

HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min.
Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody (GTX112584) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HHIP antibody [N3C2], Internal used in Western Blot (WB). GTX112584

GTX112584 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HHIP antibody [N3C2], Internal used in Western Blot (WB). GTX112584

GTX112584 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal (GTX112584) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Kosla J et al. Gene 2013; 513 (1) : 90-100 Molecular analysis of the TGF-beta controlled gene expression program in chicken embryo dermal myofibroblasts.
Application : WB
Reactivity : Chicken
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169