GeneTex
United States (US)

HIBADH antibody

GTX122994_40625_WB_18121410_780.jpg
GTX122994_40625_IHC_18121410_754.jpg
GTX122994_40625_WB_M_18121410_265.jpg
GTX122994_40625_WB_R_18121410_262.jpg

Cat No. GTX122994

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A549 , Neuro2A , C8D30 , PC-12

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIBADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

3-Hydroxyisobutyrate Dehydrogenase,Ns5Atp1,Hibadh

Cellular Localization

Mitochondrion

Background

3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX122994_40625_WB_18121410_780.jpg

GTX122994 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX122994 diluted at 1:10000

GTX122994_40625_IHC_18121410_754.jpg

GTX122994 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using HIBADH(GTX122994) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX122994_40625_WB_M_18121410_265.jpg

GTX122994 WB Image

HIBADH antibody detects HIBADH protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HIBADH antibody (GTX122994) dilution: 1:5000

GTX122994_40625_WB_R_18121410_262.jpg

GTX122994 WB Image

HIBADH antibody detects HIBADH protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10 % SDS-PAGE
HIBADH antibody (GTX122994) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169