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HIBADH antibody

Anti-HIBADH antibody used in Western Blot (WB). GTX122994
Anti-HIBADH antibody used in IHC (Paraffin sections) (IHC-P). GTX122994
Anti-HIBADH antibody used in Western Blot (WB). GTX122994
Anti-HIBADH antibody used in Western Blot (WB). GTX122994

Cat. No. GTX122994

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HIBADH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A549 , Neuro2A , C8D30 , PC-12

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIBADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169604

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxyisobutyrate dehydrogenase , NS5ATP1

Cellular Localization

Mitochondrion

Background

3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HIBADH antibody used in Western Blot (WB). GTX122994

GTX122994 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX122994 diluted at 1:10000

Anti-HIBADH antibody used in IHC (Paraffin sections) (IHC-P). GTX122994

GTX122994 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using HIBADH(GTX122994) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HIBADH antibody used in Western Blot (WB). GTX122994

GTX122994 WB Image

HIBADH antibody detects HIBADH protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HIBADH antibody (GTX122994) dilution: 1:5000

Anti-HIBADH antibody used in Western Blot (WB). GTX122994

GTX122994 WB Image

HIBADH antibody detects HIBADH protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
HIBADH antibody (GTX122994) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169