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HIBADH antibody

Anti-HIBADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64844
Anti-HIBADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64844
Anti-HIBADH antibody used in IHC (Paraffin sections) (IHC-P). GTX64844
Anti-HIBADH antibody used in IHC (Paraffin sections) (IHC-P). GTX64844
Anti-HIBADH antibody used in Western Blot (WB). GTX64844
Anti-HIBADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64844

Cat. No. GTX64844

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HIBADH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:3000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-336 of human HIBADH (NP_689953.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxyisobutyrate dehydrogenase , NS5ATP1

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-HIBADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64844

GTX64844 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64844 HIBADH antibody.
Blue : DAPI
Dilution : 1:100

Anti-HIBADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64844

GTX64844 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64844 HIBADH antibody.
Blue : DAPI
Dilution : 1:100

Anti-HIBADH antibody used in IHC (Paraffin sections) (IHC-P). GTX64844

GTX64844 IHC-P Image

IHC-P analysis of human esophageal tissue using GTX64844 HIBADH antibody.
Dilution : 1:100

Anti-HIBADH antibody used in IHC (Paraffin sections) (IHC-P). GTX64844

GTX64844 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX64844 HIBADH antibody.
Dilution : 1:100

Anti-HIBADH antibody used in Western Blot (WB). GTX64844

GTX64844 WB Image

WB analysis of various sample lysates using GTX64844 HIBADH antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-HIBADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64844

GTX64844 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64844 HIBADH antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Meyer M et al. J Inherit Metab Dis 2021; (Epub) 3-Hydroxyisobutyrate dehydrogenase (HIBADH) deficiency-A novel disorder of valine metabolism.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399