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HIBCH antibody [3H5]

Overexpression
Anti-HIBCH antibody [3H5] used in Western Blot (WB). GTX84364
Anti-HIBCH antibody [3H5] used in Flow cytometry (FACS). GTX84364
Anti-HIBCH antibody [3H5] used in IHC (Paraffin sections) (IHC-P). GTX84364
Anti-HIBCH antibody [3H5] used in IHC (Paraffin sections) (IHC-P). GTX84364
Anti-HIBCH antibody [3H5] used in Western Blot (WB). GTX84364
Anti-HIBCH antibody [3H5] used in IHC (Paraffin sections) (IHC-P). GTX84364

Cat. No. GTX84364

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3H5

Isotype

 IgG2a

Application

 WB, IHC-P, FACS

Reactivity

 Human, Dog
Package
100 μl ($519)
See all HIBCH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxyisobutyryl-CoA hydrolase , HIBYLCOAH

Cellular Localization

Mitochondrion

Background

Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.

Database

Research Area

DATA IMAGES

Anti-HIBCH antibody [3H5] used in Western Blot (WB). GTX84364

GTX84364 WB Image

Overexpression

WB analysis of HEK293T cells transfected with HIBCH plasmid (Right) or empty vector (Left) for 48 hrs using GTX84364 HIBCH antibody [3H5].
Loading : 5 ug per lane

Anti-HIBCH antibody [3H5] used in Flow cytometry (FACS). GTX84364

GTX84364 FACS Image

FACS analysis of HEK293T cells transfected with either HIBCH plasmid(Red) or empty vector control plasmid(Blue) using GTX84364 HIBCH antibody [3H5].

Anti-HIBCH antibody [3H5] used in IHC (Paraffin sections) (IHC-P). GTX84364

GTX84364 IHC-P Image

IHC-P analysis of human liver tissue using GTX84364 HIBCH antibody [3H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-HIBCH antibody [3H5] used in IHC (Paraffin sections) (IHC-P). GTX84364

GTX84364 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84364 HIBCH antibody [3H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-HIBCH antibody [3H5] used in Western Blot (WB). GTX84364

GTX84364 WB Image

WB analysis of various cell lines using GTX84364 HIBCH antibody [3H5].
Loading : 35 ug per lane

Anti-HIBCH antibody [3H5] used in IHC (Paraffin sections) (IHC-P). GTX84364

GTX84364 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84364 HIBCH antibody [3H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519