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HIBCH antibody

Anti-HIBCH antibody used in Western Blot (WB). GTX109167
Anti-HIBCH antibody used in IHC (Paraffin sections) (IHC-P). GTX109167
Anti-HIBCH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109167

Cat. No. GTX109167

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HIBCH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , HepG2 , Molt-4

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HIBCH Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950489

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxyisobutyryl-CoA hydrolase , HIBYLCOAH

Cellular Localization

Mitochondrion

Background

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HIBCH antibody used in Western Blot (WB). GTX109167

GTX109167 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
C: Molt-4
10% SDS PAGE
GTX109167 diluted at 1:1000

Anti-HIBCH antibody used in IHC (Paraffin sections) (IHC-P). GTX109167

GTX109167 IHC-P Image

HIBCH antibody detects HIBCH protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
HIBCH antibody (GTX109167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HIBCH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109167

GTX109167 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using HIBCH(GTX109167) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169