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HIC5 antibody [N1], N-term

Anti-HIC5 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108510
Anti-HIC5 antibody [N1], N-term used in Western Blot (WB). GTX108510

Cat. No. GTX108510

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HIC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

TGFB1I1 transfected 293T

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIC5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952224

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transforming growth factor beta 1 induced transcript 1 , ARA55 , HIC-5 , HIC5 , TSC-5

Cellular Localization

Cell junction , focal adhesion , Nucleus matrix , Cytoplasm , cytoskeleton

Background

This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIC5 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108510

GTX108510 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using HIC5(GTX108510) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-HIC5 antibody [N1], N-term used in Western Blot (WB). GTX108510

GTX108510 WB Image

Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 polyclonal antibody.
A: Non-transfected lysate.
B: TGFB1I1 transfected lysate.
12% SDS PAGE
GTX108510 diluted at 1:500

REFERENCE

Application Reference

Wu WS et al. Am J Cancer Res 2023; 13 (10) : 4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169