GeneTex
United States (US)

HICE1 antibody

KO/KD
GTX100735_IP_GK_19011506_742.jpg
GTX100735_WB_GK_19011506_451.jpg
GTX100735_IHC1_19011506_272.jpg
GTX100735_WB_19011506_387.jpg
GTX100735_IHC2_19011506_560.jpg
GTX100735_IF1_19011506_948.jpg

Cat No. GTX100735

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:50-1:500
IP 1:100-1:500
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

K562 , HeLa
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human HICE1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DGT4 antibody, HICE1 antibody, MGC20533 antibody, NY-SAR-48 antibody, HAUS8 antibody, "HEC1/NDC80 interacting, centrosome associated 1 antibody", Hec1-interacting and centrosome-associated 1 antibody, sarcoma antigen NY-SAR-48 antibody, HEC1/NDC80-interacting centrosome-associated protein 1 antibody, HAUS augmin-like complex subunit 8 antibody, "HAUS augmin-like complex, subunit 8 antibody"

Cellular Localization

Cytoplasm , Centrosome , Spindle

Background

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100735_IP_GK_19011506_742.jpg

GTX100735 IP Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.

GTX100735_WB_GK_19011506_451.jpg

GTX100735 WB Image

KO/KD

WB to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using GTX100735 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100735_IHC1_19011506_272.jpg

GTX100735 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (GTX100735) antibody at 1:50 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100735_WB_19011506_387.jpg

GTX100735 WB Image

Sample
A: His-Hice1 (2x)
B: His-Hice1 (1x)
7.5% SDS PAGE
GTX100735 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100735_IHC2_19011506_560.jpg

GTX100735 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (GTX100735) antibody at 1:50 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100735_IF1_19011506_948.jpg

GTX100735 ICC/IF Image

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1(GTX100735) antibody at 1:50-1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169