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HICE1 antibody

KO/KD
Anti-HICE1 antibody used in Immunoprecipitation (IP). GTX100735
Anti-HICE1 antibody used in Western Blot (WB). GTX100735
Anti-HICE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100735
Anti-HICE1 antibody used in Western Blot (WB). GTX100735
Anti-HICE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100735
Anti-HICE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100735

Cat. No. GTX100735

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HICE1 products
PRODUCT

Summary

HICE1 antibody recognizes HICE1 protein encoded by the human HAUS8 gene. HICE1 is a subunit of the microtubule-associated HAUS augmin-like complex, which is involved in maintenance of spindle integrity and microtubule nucleation during spindle assembly. Disruption of HICE1 results in abnormal spindle configuration, mitotic delay, and chromosomal instability.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:50-1:500
IP 1:100-1:500
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

U2OS

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human HICE1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950490

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HAUS augmin like complex subunit 8 , DGT4 , HICE1 , NY-SAR-48

Cellular Localization

Cytoplasm , Centrosome , Spindle

Background

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HICE1 antibody used in Immunoprecipitation (IP). GTX100735

GTX100735 IP Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.

Anti-HICE1 antibody used in Western Blot (WB). GTX100735

GTX100735 WB Image

KO/KD

WB to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using GTX100735 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HICE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100735

GTX100735 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (GTX100735) antibody at 1:50 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HICE1 antibody used in Western Blot (WB). GTX100735

GTX100735 WB Image

Sample
A: His-Hice1 (2x)
B: His-Hice1 (1x)
7.5% SDS PAGE
GTX100735 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HICE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100735

GTX100735 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (GTX100735) antibody at 1:50 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HICE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100735

GTX100735 ICC/IF Image

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1(GTX100735) antibody at 1:50-1:200 dilution.

REFERENCE

Application Reference

Johmura Y et al. Proc Natl Acad Sci U S A 2011; 108 (28) : 11446-51 Regulation of microtubule-based microtubule nucleation by mammalian polo-like kinase 1.
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169