APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-term region of human HIF1 alpha. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78
Cellular Localization
Cytoplasm,Nucleus,Nucleus speckle
Background
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Database
Research Area
DATA IMAGES
![Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766](/templates/default/js/lightbox/images/loading.gif "Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766")
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GTX629766 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT122] (GTX629766) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX629766 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT122] (GTX629766) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629766 ICC/IF Image
HIF1 alpha antibody [GT122] detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody [GT122] (GTX629766) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for HIF1 alpha antibody [GT122] (GTX629766). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HIF1 alpha antibody [GT122] (GTX629766). Be the first to share your experience with this product.
![Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766](/upload/website/prouct_img/small/GTX629766/GTX629766_44615_20220318_WB_M_treatment_CoCl2_w_23051500_576.webp "Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766")
![Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766](/upload/website/prouct_img/small/GTX629766/GTX629766_44615_20220318_WB_treatment_CoCl2_w_23051500_146.webp "Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766")
![Anti-HIF1 alpha antibody [GT122] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629766](/upload/website/prouct_img/small/GTX629766/GTX629766_44615_20220429_ICC_IF_treatment_CoCl2_w_23051500_876.webp "Anti-HIF1 alpha antibody [GT122] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629766")
![Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766](/upload/website/prouct_img/normal/GTX629766/GTX629766_44615_20220318_WB_M_treatment_CoCl2_w_23051500_576.webp "Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766")
![Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766](/upload/website/prouct_img/normal/GTX629766/GTX629766_44615_20220318_WB_treatment_CoCl2_w_23051500_146.webp "Anti-HIF1 alpha antibody [GT122] used in Western Blot (WB). GTX629766")
![Anti-HIF1 alpha antibody [GT122] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629766](/upload/website/prouct_img/normal/GTX629766/GTX629766_44615_20220429_ICC_IF_treatment_CoCl2_w_23051500_876.webp "Anti-HIF1 alpha antibody [GT122] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629766")