HIF1 alpha antibody [H1alpha67]

Cat. No. GTX30105

Reference 9

Host	Mouse
Clonality	Monoclonal
Clone Name	H1alpha67
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Activation, Gel supershift assays, IHC
Reactivity	Human, Mouse, Rat, Yeast, Rabbit, Sheep, Bovine, Pig, Monkey, Bat, Ferret, Xenopus, Primate
Package	100 μl ($449)

See all HIF1 alpha products

View product citations for antibody GTX30105 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:50
IHC-P	1:20 - 1:50
IHC-Fr	1:20 - 1:50
FACS	1:10 - 1:1000
IP	1:10 - 1:500
ELISA	1:100 - 1:2000
ChIP assay	1 - 5 μg
Activation	Assay dependent
Gel supershift assays	1:1 - 1:100
IHC	1:20 - 1:50

Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm,Nucleus,Nucleus speckle

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Gene ID: 3091 HIF1A
UniProt: Q16665 HIF1A

Research Area

DATA IMAGES

GTX30105 IHC-P Image

IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].

GTX30105 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].

GTX30105 ICC/IF Image

ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67].
Red : primary antibody
Blue : DAPI
Dilution : 1 μg/ml