GeneTex
United States (US)

HIF1 alpha antibody [H1alpha67]

Orthogonal
GTX30105_1324_WB_18121410_335.jpg
GTX30105_308_IHC-P_18121410_733.jpg
GTX30105_532_IHC-P_18121410_502.jpg
GTX30105_174_ICC-IF_18121410_869.jpg

Cat No. GTX30105

Host Mouse
Clonality Monoclonal
Clone Name H1alpha67
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Activation, GSA, IHC
Reactivity Human, Mouse, Rat, Yeast, Rabbit, Sheep, Bovine, Pig, Monkey, Bat, Ferret, Xenopus, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:50
IHC-P 1:20 - 1:50
IHC-Fr 1:20 - 1:50
FACS 1:10 - 1:1000
IP 1:10 - 1:500
ELISA 1:100 - 1:2000
ChIP assay 1 - 5 μg
Activation Assay dependent
GSA 1:1 - 1:100
IHC 1:20 - 1:50
Not tested in other applications.

Calculated MW

93 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypoxia Inducible Factor 1 Subunit Alpha,Hif-1-Alpha,Hif-1A,Hif-1Alpha,Hif1,Hif1-Alpha,Mop1,Pasd8,Bhlhe78,Hif1A

Cellular Localization

Cytoplasm,Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30105_1324_WB_18121410_335.jpg

GTX30105 WB Image

Orthogonal

WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67].
Loading : 50μg

GTX30105_308_IHC-P_18121410_733.jpg

GTX30105 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].

GTX30105_532_IHC-P_18121410_502.jpg

GTX30105 IHC-P Image

IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].

GTX30105_174_ICC-IF_18121410_869.jpg

GTX30105 ICC/IF Image

ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67].
Red : primary antibody
Blue : DAPI
Dilution : 1 μg/ml

REFERENCE