GeneTex
  • Country / Location Selection

United States (US)

HIF1 alpha antibody [H1alpha67]

Anti-HIF1 alpha antibody [H1alpha67] used in Western Blot (WB). GTX30105
Anti-HIF1 alpha antibody [H1alpha67] used in IHC (Paraffin sections) (IHC-P). GTX30105
Anti-HIF1 alpha antibody [H1alpha67] used in IHC (Paraffin sections) (IHC-P). GTX30105
Anti-HIF1 alpha antibody [H1alpha67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30105

Cat No. GTX30105

Host

Mouse

Clonality

Monoclonal

Clone Name

H1alpha67

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Activation, Gel supershift assays, IHC

Reactivity

Human, Mouse, Rat, Yeast, Rabbit, Sheep, Bovine, Pig, Monkey, Bat, Ferret, Xenopus, Primate
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:50
IHC-P 1:20 - 1:50
IHC-Fr 1:20 - 1:50
FACS 1:10 - 1:1000
IP 1:10 - 1:500
ELISA 1:100 - 1:2000
ChIP assay 1 - 5 μg
Activation Assay dependent
Gel supershift assays 1:1 - 1:100
IHC 1:20 - 1:50
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372307

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm,Nucleus,Nucleus speckle

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody [H1alpha67] used in Western Blot (WB). GTX30105

GTX30105 WB Image

WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67].
Loading : 50μg

Anti-HIF1 alpha antibody [H1alpha67] used in IHC (Paraffin sections) (IHC-P). GTX30105

GTX30105 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].

Anti-HIF1 alpha antibody [H1alpha67] used in IHC (Paraffin sections) (IHC-P). GTX30105

GTX30105 IHC-P Image

IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].

Anti-HIF1 alpha antibody [H1alpha67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30105

GTX30105 ICC/IF Image

ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67].
Red : primary antibody
Blue : DAPI
Dilution : 1 μg/ml

REFERENCE

REVIEW

There are currently no reviews for HIF1 alpha antibody [H1alpha67] (GTX30105). Be the first to share your experience with this product.
Application Reference
SDS
PBS.pdf
Sodium Azide.pdf