GeneTex
United States (US)

HIF1 alpha antibody [H1alpha67]

Orthogonal
GTX30115_1330_WB_18121410_262.jpg
GTX30115_310_IHC-P_18121410_884.jpg

Cat No. GTX30115

Host Mouse
Clonality Monoclonal
Clone Name H1alpha67
Isotype IgG2b
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, GSA
Reactivity Human, Mouse, Rat, Sheep, Bovine, Dog, Pig, Monkey, Ferret, Primate, Avian
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:10 - 1:500
IHC-P 1:100 - 1:300
FACS 1:10 - 1:1000
IP 1:10
ELISA 1:100 - 1:2000
ChIP assay 1:10 - 1:500
GSA 1:1 - 1:100
Not tested in other applications.

Calculated MW

93 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIF-1-alpha antibody , HIF-1A antibody , HIF-1alpha antibody , HIF1 antibody , HIF1-ALPHA antibody , MOP1 antibody , PASD8 antibody , bHLHe78 antibody

Cellular Localization

Cytoplasm,Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30115_1330_WB_18121410_262.jpg

GTX30115 WB Image

Orthogonal

WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30115 HIF1 alpha antibody [H1alpha67].

GTX30115_310_IHC-P_18121410_884.jpg

GTX30115 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30115 HIF1 alpha antibody [H1alpha67].

REFERENCE