GeneTex
United States (US)

HIF1 alpha antibody [RM242]

Anti-HIF1 alpha antibody [RM242] used in IHC (Paraffin sections) (IHC-P). GTX33617
Anti-HIF1 alpha antibody [RM242] used in Western Blot (WB). GTX33617

Cat No. GTX33617

Host Rabbit
Clonality Monoclonal
Clone Name RM242
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Rat
APPLICATION

Application Note

Immunohistochemistry (IHC): 1:500-1:1000 dilution, Western Blot (WB): 1:1000-1:2000 dilution.

Calculated MW

93 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to human HIF-1-alpha (Hypoxia-inducible factor 1-alpha).
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to Hypoxia-inducible factor 1-alpha

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypoxia Inducible Factor 1 Subunit Alpha,Hif-1-Alpha,Hif-1A,Hif-1Alpha,Hif1,Hif1-Alpha,Mop1,Pasd8,Bhlhe78,Hif1A

Background

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES
Anti-HIF1 alpha antibody [RM242] used in IHC (Paraffin sections) (IHC-P). GTX33617

GTX33617 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-HIF-1-alpha RM242at a 1:1000 dilution.

Anti-HIF1 alpha antibody [RM242] used in Western Blot (WB). GTX33617

GTX33617 WB Image

Western Blot of Jurkat cell lysate, nontreated or treated with Cobalt(II) chloride (CoCl2), using Anti-HIF-1-alpha RM242 at a 1:1000 dilution, showed that HIF-1-alpha (~120 kDa) expressionwas induced by CoCl2 in Jurkat cells.

REFERENCE