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HIF1 alpha antibody [RM242]

Orthogonal
Anti-HIF1 alpha antibody [RM242] used in IHC (Paraffin sections) (IHC-P). GTX33617
Anti-HIF1 alpha antibody [RM242] used in Western Blot (WB). GTX33617

Cat. No. GTX33617

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM242

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all HIF1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
IHC-P 1:500 - 1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to Hypoxia-inducible factor 1-alpha

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887727

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HIF1,HIF1A,HIF1ALPHA,HIF1alpha,MOP1,PASD8,bHLHe78,hypoxia inducible factor 1 alpha subunit,HIF1 alpha

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody [RM242] used in IHC (Paraffin sections) (IHC-P). GTX33617

GTX33617 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX33617 HIF1 alpha antibody [RM242].
Dilution : 1:1000

Anti-HIF1 alpha antibody [RM242] used in Western Blot (WB). GTX33617

GTX33617 WB Image

Orthogonal

WB analysis of Jurkat cell nontreated or treated with Cobalt(II) chloride (CoCl2) lysates using GTX33617 HIF1 alpha antibody [RM242].
Dilution : 1:1000

REFERENCE

Application Reference

Gonzalez-Pacheco H et al. ScientificWorldJournal 2014; 2014 : 187071 Pre-conditioning with CDP-choline attenuates oxidative stress-induced cardiac myocyte death in a hypoxia/reperfusion model.
Application : WB
Reactivity : Rat
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REVIEW

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Package List Price ($)
$ 399