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HIF1 alpha antibody

KO/KD Comparison Orthogonal Overexpression
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

Cat. No. GTX127309

Reference 172
64

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine
Package
100 μl ($399),
25 μl ($169)
See all HIF1 alpha products
PRODUCT

Summary

HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a master regulator of homeostatic adaption to hypoxia (low oxygen supply) in various physiological and pathological conditions including normal development and cancer.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:570
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HepG2 (1% O2 treatment for 24hr) , 293T transfected HIF1A

Product Note

Knockdown/Knockout validation was supported by references (PMID:25428606)

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616089

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm , Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

KO/KD

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309

GTX127309 IHC-P Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody (GTX127309) dilution: 1:500.
Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309

GTX127309 ChIP assay Image

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309

GTX127309 IP Image

Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309).
Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) Rat-2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chang HA et al. Cell Death Discov 2023; 9 (1) : 385 YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions.
M Horie et al. Mol Oncol 2023; Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment
Yan J et al. Chin Med 2023; 18 (1) : 90 LuQi Formula relieves ventricular remodeling through improvement of HIF-1α-mediated intestinal barrier integrity.
Qin X et al. J Biol Chem 2023; 299 (8) : 105071 The disordered C terminus of ALKBH5 promotes phase separation and paraspeckles assembly.
S?nchez-Gloria JL et al. Int J Mol Sci 2023; 24 (16) Allicin, an Emerging Treatment for Pulmonary Arterial Hypertension: An Experimental Study.
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REVIEW

HIF1 alpha antibody Cat. No. GTX127309

Rating
( Average 4.7 based on 64 users reviews)
Application
Western Blot(WB)
( Average 4.8 based on 54 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4.2 based on 6 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the 2022 in Open Life Sci. PMID: 35291563
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Pharmacol. PMID: 35222025
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Front Pharmacol. PMID: 35222025
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Sci Rep. PMID: 35027586
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the 2019 in Aging (Albany NY). PMID: 31584878
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Int J Mol Sci. PMID: 35328751
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Int J Mol Sci. PMID: 30609861
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Int J Mol Sci. PMID: 30609861
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Int J Mol Sci. PMID: 30609861
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2019 in Int J Mol Sci. PMID: 30609861
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 10-Feb-2022
Score :
Application Tested : IHC-P
Sample Species : Ms, Hu
Sample : Human and mouse liver tissue sections
Blocking : goat serum
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Citrate Buffer, 0.25Hr
Primary Antibody : 1:200, 4°C, 12Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Dec-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human pancreatic cancer cell line
Amount used : 5μg
Blocking : 3% Skim milk/TBS-T, 24°C, 1Hr
Primary Antibody : 1:2000, 4°C, 20Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Nov-2021
Score :
Application Tested : WB
Sample Species : Bov
Sample : Bovine brain capillary endothelial cells
Amount used : 40μg
Blocking : Skim milk, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 29121675
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28639619
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28639619
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Pharmaceuticals (Basel) in 2018.PMID: 30487460
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2018.PMID: 29533007
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Med in 2018.PMID: 29533007
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 19-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco-2 cells
Amount used : 10 μg
Blocking : 5 % Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 27-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse fibroblast cell lyate
Blocking : 5% non-fat milk in TBST, 28°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Cytosol fraction of untreated cells
Lane 2: Cytosol fraction of CoCl2 treated cells (200 uM, 48 hr)
Lane 3: blank
Lane 4: Mito/Nucleus fraction of untreated cells
Lane 5: Mito/Nucleus fraction of CoCl2 treated cells (200 uM, 48 hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 25-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A375 cells
Blocking : 5% BSA in TBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27355368
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2013. PMID: 24423398
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28775317
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019. PMID: 31174567
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 26868506
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28775317
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28775317
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cells in 2019. PMID: 31905895
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30796225
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29721188
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29383096
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27764809
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27557492
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29383096
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27557492
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28617871
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29137374
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Sep-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : LoVo cells
Blocking : 3% skim-milk in TBST, 4°C, 18Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : LoVo cell were cultured using the hypoxic culture kit under hypoxic culture conditions (1% O2 at 37ºC) for 1, 2, 3, or 4 d. Lysates of normoxic and hypoxic cultured cells were separated on 8% SDS-PAGE gels and were immunoblotted using HIF-1 alpha Ab.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Sep-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : DLD-1 cells
Blocking : 3% skim-milk in TBST, 4°C, 18Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : DLD-1 cell were cultured using the hypoxic culture kit under hypoxic culture conditions (1% O2 at 37ºC) for 1, 2, 3, or 4 d. Lysates of normoxic and hypoxic cultured cells were separated on 8% SDS-PAGE gels and were immunoblotted using HIF-1 alpha Ab.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 25-Apr-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : RAW264.7 cells
Amount used : 20 μg
Blocking : 3% non-fat dry milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-HIF1 alpha antibody used in IHC (Frozen sections) (IHC-Fr). GTX127309
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Adult cardiac tissue
Blocking : 10% goat serum in PBS-TX100 0.1%, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton-X-100/Citrate Buffer pH6, 0.15Hr
Primary Antibody : 1:50, 4°C, 16Hr
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Renal clear cell carcinoma
Blocking : EnVISION FLEX PEROXIDASE-BLOCKING REAGENT (Dako, Ref. K800)
Fixation : 3.7% formalin
Permeabilization /Antigen retrieval : pH 9 Envision FLEX target retrieval solution, High pH, (Dako Ref. GV800)
Primary Antibody : 1:100, 25°C, 1Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10 μg
Blocking : ATTO EzBlock Chemi, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-P
Sample : HeLa xenograft
Blocking : 10% goat serum, 25°C, 0.08Hr
Fixation : Formalin Neutral Buffer
Permeabilization /Antigen retrieval : 0.2% Brij35 in PBS/Dako S2369
Primary Antibody : 1:100, 25°C, 0.6Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Vehicle, Lane 2: 500 μM Cocl2 treatment for 4 hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Normoxia, Lane 2: 1% O2 treatment for 4 hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 22-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : dental pulp stem cells
Amount used : 1x10^4 cells
Blocking : PVDF blocking reagent, 25°C, 0.25Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 02-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1:THP-1 cells (250ug/ml TPA treatment for 4h), Lane 2:THP-1 cells (250ug/ml TPA and 1 ug/ml LPS treatment for 4h)
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A: A549 cells, B: A549 cells (1mM DMOG treatment for 3.5 h)
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : brain tissue
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A:Human glioblastoma tissue, B:Human healthy brain tissue
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : meniscal fibrochondrocytes
Amount used : 15μg
Blocking : BlockingPRO Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: meniscal fibrochondrocytes (normoxia), Lane 2: meniscal fibrochondrocytes (1% O2 treatment for 6hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : endometriotic stromal cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane A：DMSO treatment (24hr) ; Lane B：0.1uM DFO treatment (24hr) ; Lane C：1uM DFO treatment (24hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane A: HeLa cell lysate(normoxia), Lane B: HeLa cell lysate (500μM CoCl2 treatment)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 37°C, 1Hr
Primary Antibody : 1:1000, 37°C, 1Hr
Lane Description : A: A431 whole cell lysate under normoxia, B: A431 whole cell lysate under hypoxia
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 16-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 3% milk , 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
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Application Reference
Chang HA et al. Cell Death Discov 2023; 9 (1):385 YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions.
M Horie et al. Mol Oncol 2023; Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment
Yan J et al. Chin Med 2023; 18 (1):90 LuQi Formula relieves ventricular remodeling through improvement of HIF-1α-mediated intestinal barrier integrity.
Qin X et al. J Biol Chem 2023; 299 (8):105071 The disordered C terminus of ALKBH5 promotes phase separation and paraspeckles assembly.
S?nchez-Gloria JL et al. Int J Mol Sci 2023; 24 (16) Allicin, an Emerging Treatment for Pulmonary Arterial Hypertension: An Experimental Study.
AJ Little et al. JCI Insight 2023;:e166076 HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease
Application : IHC-Fr
Reactivity : Mouse
Chen J et al. J Orthop Translat 2023; 40:49-57 HIF-1α dependent RhoA as a novel therapeutic target to regulate rheumatoid arthritis fibroblast-like synoviocytes migration in vitro and in vivo.
Csiki DM et al. Front Cardiovasc Med 2023; 10:1168339 Hypoxia-inducible factor activation promotes osteogenic transition of valve interstitial cells and accelerates aortic valve calcification in a mice model of chronic kidney disease.
Garc?a-Arroyo FE et al. Antioxidants (Basel) 2023; 12 (5) Osthole Prevents Heart Damage Induced by Diet-Induced Metabolic Syndrome: Role of Fructokinase (KHK).
Wu SK et al. Aging Cell 2023; 22 (3):e13766 The variant senescence-associated secretory phenotype induced by centrosome amplification constitutes a pathway that activates hypoxia-inducible factor-1α.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Ito R et al. Am J Physiol Lung Cell Mol Physiol 2022; 323 (2):L129-L141 SM22α cell-specific HIF stabilization mitigates hyperoxia-induced neonatal lung injury.
Chai X et al. Transl Cancer Res 2022; 11 (6):1644-1651 Expression of HIF-1α, ANXA3, CD133 and their associations with clinicopathological parameters in human colon carcinoma.
Application : IHC-P
Reactivity : Human
Meng X et al. Oncogene 2022; 41 (36):4231-4243 Roles of lncRNA LVBU in regulating urea cycle/polyamine synthesis axis to promote colorectal carcinoma progression.
Application : ChIP assay
Reactivity : Human
Zhang Y et al. Molecules 2022; 27 (21)
Bachmann M et al. Cell Death Dis 2022; 13 (12):1055 Pharmacological targeting of the mitochondrial calcium-dependent potassium channel KCa3.1 triggers cell death and reduces tumor growth and metastasis in vivo.
Application : WB
Reactivity : Mouse
Huang YJ et al. Cancers (Basel) 2022; 14 (16) HIF-1α Expression Increases Preoperative Concurrent Chemoradiotherapy Resistance in Hyperglycemic Rectal Cancer.
Application : WB, IHC-P
Reactivity : Human
Qu X et al. Anal Cell Pathol (Amst) 2022; 2022:3066456 XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps.
Application : WB
Reactivity : Mouse
Hsing-Hua Chao, Jun-Liang Luo et al. Cell Death Dis . 2022; 13 (9) Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells
Application : WB, ChIP assay
Reactivity : Human
Hsing-I Tseng et al. Mol Oncol 2022; 16 (11):2274-2294 A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α-mediated aerobic glycolysis.
Application : WB, IHC-P
Reactivity : Human
Go Itoh et al. Mol Oncol 2022; 16 (1):166-187 Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T-cell suppression.
Application : WB
Xiaowei Zheng et al. Elife 2022; 11 Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Jiapeng Li et al. Open Life Sci 2022; 17 (1):121-130 Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway.
Application : IHC-P
Reactivity : Mouse
Shion Orikasa et al. Sci Rep 2022; 12 (1):682 Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
Application : WB
Reactivity : Human
Izabela Bary?a et al. Int J Mol Sci 2022; 23 (6) The WWOX/HIF1A Axis Downregulation Alters Glucose Metabolism and Predispose to Metabolic Disorders.
Application : WB
Reactivity : Human
Bingchuan Geng et al. Redox Biol 2022; 52:102295 UCHL1 protects against ischemic heart injury via activating HIF-1α signal pathway.
Application : WB, IP
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Andrea T?th et al. Front Pharmacol 2022; 13:798053 Daprodustat Accelerates High Phosphate-Induced Calcification Through the Activation of HIF-1 Signaling.
Application : WB
Reactivity : Human
YF Yang et al. et al. J Cell Mol Med 2022; UBE2C triggers HIF-1α-glycolytic flux in head and neck squamous cell carcinoma
Application : WB
Reactivity : Human
Xiaohu Huang et al. Cancers (Basel) 2021; 13 (4) Dual Targeting Oncoproteins MYC and HIF1α Regresses Tumor Growth of Lung Cancer and Lymphoma.
Application : WB
Reactivity : Human
Bo Yan et al. Transl Androl Urol 2021; 10 (5):2051-2066 The protective effects of exogenous spermine on renal ischemia-reperfusion injury in rats.
Application : WB
Reactivity : Human
Chen Zhang et al. Int J Med Sci 2021; 18 (15):3403-3411 Cobalt chloride-stimulated hypoxia promotes the proliferation of cholesteatoma keratinocytes via the PI3K/Akt signaling pathway.
Application : WB, IHC-P
Reactivity : Human
Yi-Chih Tsai et al. Exp Mol Med 2021; 53 (10):1623-1635 Upregulating sirtuin 6 ameliorates glycolysis, EMT and distant metastasis of pancreatic adenocarcinoma with kr?ppel-like factor 10 deficiency.
Application : WB
Reactivity : Human
Giovannucci TA et al. Autophagy 2021;:1-17 Identification of a novel compound that simultaneously impairs the ubiquitin-proteasome system and autophagy.
Application : WB
Reactivity : Human
Shih HJ et al. Sci Rep 2021; 11 (1):22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Application : WB, ICC/IF
Reactivity : Human
Owada S et al. Anticancer Res 2021; 41 (12):6051-6059 Evaluation of Erastin as a Therapeutic Agent Under Hypoxic Conditions in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Peng G et al. J Exp Clin Cancer Res 2021; 40 (1):278 The HIF1α-PDGFD-PDGFRα axis controls glioblastoma growth at normoxia/mild-hypoxia and confers sensitivity to targeted therapy by echinomycin.
Application : WB
Reactivity : Human
Chen YC et al. Cell Biol Toxicol 2021; (Epub) Vitamin D3 decreases TNF-α-induced inflammation in lung epithelial cells through a reduction in mitochondrial fission and mitophagy.
Application : WB
Reactivity : Human
N?chel J et al. Mol Cell 2021; 81 (16):3275-3293.e12 An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
Takenaga K et al. Sci Rep 2021; 11 (1):13302 MCT4 is induced by metastasis-enhancing pathogenic mitochondrial NADH dehydrogenase gene mutations and can be a therapeutic target.
Application : WB
Reactivity : Mouse
Hsu CG et al. J Immunol 2021; Phosphodiesterase 10A Is a Key Mediator of Lung Inflammation.
Sun X et al. Front Genet 2021; 12:680928 Expression and Clinical Significance of HKII and HIF-1α in Grade Groups of Prostate Cancer.
Application : IHC-P
Reactivity : Human
Coronel-Hern?ndez J et al. Front Oncol 2021; 11:594200
Application : WB
Gampala S et al. Br J Cancer 2021; 124 (9):1566-1580 Exploring transcriptional regulators Ref-1 and STAT3 as therapeutic targets in malignant peripheral nerve sheath tumours.
Application : WB
Reactivity : Human
Nadeau JR et al. Exp Neurol 2021; 341:113671 Acute intermittent hypoxia enhances regeneration of surgically repaired peripheral nerves in a manner akin to electrical stimulation.
Ezzeddini R et al. J Physiol Biochem 2021; 77 (2):249-260 Downregulation of fatty acid oxidation by involvement of HIF-1α and PPARγ in human gastric adenocarcinoma and related clinical significance.
Application : WB
Nakashima M et al. Cancer Sci 2021; 112 (6):2542-2555 Differentiation of Hodgkin lymphoma cells by reactive oxygen species and regulation by heme oxygenase-1 through HIF-1α.
Application : ICC/IF
Reactivity : Human
Kitajima S et al. Sci Rep 2021; 11 (1):4538 A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition.
Application : WB
Reactivity : Human
Nishida D et al. Sci Rep 2021; 11 (1):4575 RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp.
Weng M et al. Front Cell Dev Biol 2021; 9:642930 Hypoxia-Induced LIN28A mRNA Promotes the Metastasis of Colon Cancer in a Protein-Coding-Independent Manner.
Application : WB, IHC-P
Reactivity : Human
Douiev L et al. Cells 2021; 10 (2) Upregulation of COX4-2 via HIF-1α in Mitochondrial COX4-1 Deficiency.
Application : ICC/IF
Reactivity : Human
Sasaki Y et al. Cancer Sci 2021; 112 (3):1251-1261 Expression of asporin reprograms cancer cells to acquire resistance to oxidative stress.
Application : WB
Reactivity : Human
Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Application : IHC-Fr
Reactivity : Mouse
Ranjala Ratnayake et al. Chembiochem 2020; 21 (16):2356-2366 Dolastatin 15 from a Marine Cyanobacterium Suppresses HIF-1α Mediated Cancer Cell Viability and Vascularization.
Application : WB
Reactivity : Human
Junjing Yin et al. Acta Pharm Sin B 2020; 10 (11):2246-2257 Phospholipid membrane-decorated deep-penetrated nanocatalase relieve tumor hypoxia to enhance chemo-photodynamic therapy.
Application : ICC/IF
Reactivity : Mouse
Sampath Narayanan et al. Commun Biol 2020; 3 (1):768 HypoxamiR-210 accelerates wound healing in diabetic mice by improving cellular metabolism.
Application : IHC-P
Reactivity : Mouse
Song E et al. bioRxiv 2020; (Epub) Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Ye D et al. Stem Cell Res Ther 2020; 11 (1):382 Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus.
Chen KC et al. Int J Mol Sci 2020; 21 (9) Bicalutamide Elicits Renal Damage by Causing Mitochondrial Dysfunction via ROS Damage and Upregulation of HIF-1.
Arellano-Buend?a AS et al. Antioxidants (Basel) 2020; 9 (11) Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes.
Application : WB
Reactivity : Rat
Cirotti C et al. EMBO Rep 2020;:e50500 Redox activation of ATM enhances GSNOR translation to sustain mitophagy and tolerance to oxidative stress.
Application : WB
Reactivity : Human
Ueshima E et al. Liver Cancer 2020; 9 (1):63-72 Hepatic Artery Embolization Induces the Local Overexpression of Transforming Growth Factor β1 in a Rat Hepatoma Model.
Chen HY et al. Chem Biol Interact 2020; 331:109249 TGF-β1 signaling protects retinal ganglion cells from oxidative stress via modulation of the HO-1/Nrf2 pathway.
Application : WB
Reactivity : Mouse
Hou J et al. Nat Cell Biol 2020; 22 (10):1264-1275 PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis.
Application : WB
Reactivity : Human
Tazawa K et al. Am J Pathol 2020; 190 (12):2417-2426 Transient Receptor Potential Ankyrin 1 Is Up-Regulated in Response to Lipopolysaccharide via P38/Mitogen-Activated Protein Kinase in Dental Pulp Cells and Promotes Mineralization.
Application : WB
Reactivity : Human
Owada S et al. Anticancer Res 2020; 40 (9):5071-5079 Setanaxib as a Potent Hypoxia-specific Therapeutic Agent Against Liver Cancer.
Application : WB
Reactivity : Human
Zhao Y et al. Am J Physiol Renal Physiol 2020; 319 (2):F345-F357 Endothelial prolyl hydroxylase 2 is necessary for angiotensin II-mediated renal fibrosis and injury.
Application : WB
Reactivity : Mouse
Bailey CM et al. Nanomedicine 2020; 29:102278 Liposomal formulation of HIF-1α inhibitor echinomycin eliminates established metastases of triple-negative breast cancer.
Application : WB
Reactivity : Human
Chan CH et al. Aging (Albany NY) 2020; 12 (18):17930-17947 Modulating redox homeostasis and cellular reprogramming through inhibited methylenetetrahydrofolate dehydrogenase 2 enzymatic activities in lung cancer.
Application : WB
Reactivity : Human
Takaki H et al. J Vasc Interv Radiol 2020; 31 (9):1475-1482.e2 Hepatic Artery Embolization Enhances Expression of Programmed Cell Death 1 Ligand 1 in an Orthotopic Rat Hepatocellular Carcinoma Model: In?Vivo and in?Vitro Experimentation.
Application : WB, IHC-P
Reactivity : Rat
Mochizuki M et al. Stem Cell Res Ther 2020; 11 (1):267 Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture.
Application : ICC/IF
Reactivity : Human
Eric Song et al. 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.
Application : IHC-Fr
Reactivity : Human
Dou YQ et al. Theranostics 2020; 10 (3):1197-1212 Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia.
Application : WB, ICC/IF, ChIP assay
Reactivity : Human
Ve?e?a J et al. Stem Cell Res 2020; 45:101770 Hypoxia/Hif1α Prevents Premature Neuronal Differentiation of Neural Stem Cells Through the Activation of Hes1.
Application : WB
Reactivity : Mouse
Gojkovic M et al. Acta Physiol (Oxf) 2020; 229 (2):e13461 Deregulated hypoxic response in myeloid cells: A model for high-altitude pulmonary oedema (HAPE).
Application : WB
Reactivity : Mouse
Kato H et al. Oncogene. 2020; (Epub) Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization.
Application : WB
Reactivity : Human
Fujii M et al. Int Endod J 2020; (Epub) Hypoxia-inducible factor 1α promotes interleukin 1β and tumour necrosis factor α expression in lipopolysaccharide-stimulated human dental pulp cells.
Application : WB
Reactivity : Human
Zhang S et al. Gene 2020; 733:144383 MicroRNA-574-5p in gastric cancer cells promotes angiogenesis by targeting protein tyrosine phosphatase non-receptor type 3 (PTPN3).
Application : WB
Reactivity : Human
Singh M et al. Cancer Res 2019; 79 (11):2962-2977 Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration.
Chen W et al. Oncol Lett 2019; 17 (3):2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Manuprasert W et al. Asian Pac J Allergy Immunol 2019; (Epub) Intermittent hypoxia in rat enhancing peritoneal membrane thickening through HIF-1α-induced cytokines in peritoneum.
Reactivity : Rat
Chua KV et al. Cells 2019; 9 (1) Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90帢 Secretion, and Tumor Growth.
Application : WB
Reactivity : Human
Wu TH et al. Int J Mol Sci 2019; 20 (23)
Application : WB
Reactivity : Human
Pan CH et al. Aging (Albany NY) 2019; 11 (19):8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.
Application : WB
Reactivity : Rat
Zheng J et al. J Ethnopharmacol 2019;:112330 BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic mice.
Application : WB
Reactivity : Mouse
Ho YJ et al. Theranostics 2019; 9 (24):7370-7383 Normalization of Tumor Vasculature by Oxygen Microbubbles with Ultrasound.
Application : WB
Reactivity : Mouse
Li F et al. World J Pediatr 2019; The neuroprotective effect of mesenchymal stem cells is mediated through inhibition of apoptosis in hypoxic ischemic injury.
Application : WB
Reactivity : Human
Loh XY et al. Cancer Res 2019; RNA-binding protein ZFP36L1 suppresses hypoxia and cell cycle signaling.
Application : WB
Reactivity : Human
Tang K et al. Oncogene 2019; (Epub) Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.
Application : IP
Reactivity : Human
Kuo YL et al. Sci Rep 2019; 9 (1):10269 Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture.
Application : WB, ICC/IF, IHC-P
Reactivity : Human, Mouse
Lai PY et al. Physiol Genomics 2019; Adverse Early Life Environment Impairs Postnatal Lung Development in Mice.
Application : WB
Reactivity : Mouse
Offer S et al. J Exp Clin Cancer Res 2019; 38 (1):241 Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration.
Application : WB
Reactivity : Human
Holzer T et al. J Cell Biol 2019; 218 (6):1853-1870 Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases.
Application : WB
Reactivity : Mouse
Ma Z et al. Cell Cycle 2019; 18 (13):1473-1489 Deletion of clock gene Bmal1 impaired the chondrocyte function due to disruption of the HIF1帢-VEGF signaling pathway.
Application : IHC-P
Reactivity : Mouse
Zhou M et al. Sci Rep 2019; 9 (1):6035 The pro-angiogenic role of hypoxia inducible factor stabilizer * and its application in an in vivo tissue engineering chamber model.
Application : IHC-P
Reactivity : Human
Wang X et al. J Steroid Biochem Mol Biol 2019; 191:105370 Transcription regulation of NRF1 on StAR reduces testosterone synthesis in hypoxemic murine.
Application : WB
Reactivity : Mouse
Song S et al. Am J Transl Res 2019; 11 (4):2155-2167 Blocking transforming growth factor-beta reduces the migration and invasion of the residual tumour after TAE.
Application : IHC-Fr
Reactivity : Rabbit
Ezzeddini R et al. Life Sci 2019; 224:169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.
Application : WB
Reactivity : Human
Kurelac I et al. Nat Commun 2019; 10 (1):903 Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.
Application : WB
Reactivity : Human
Wenli Chen et al. Oncology Letters 2019;:2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Application : WB, IHC-P
Yasunaga M et al. Cancer Sci 2019; 110 (2):761-770 Significant antitumor effect of an antibody against TMEM180, a new colorectal cancer-specific molecule.
Application : WB
Reactivity : Human
Hsu HL et al. Int J Mol Sci 2019; 20 (1) Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.
Application : WB
Reactivity : Human
Lai HH et al. J Clin Invest 2018; 128 (2):625-643 HIF-1α promotes autophagic proteolysis of Dicer and enhances tumor metastasis.
Kuriyama S et al. Cell Death Discov 2018; 4:11 PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Kitajima S et al. Oncotarget 2018; 9 (27):19123-19135 Hypoxia-inducible factor-2 alpha up-regulates CD70 under hypoxia and enhances anchorage-independent growth and aggressiveness in cancer cells.
Basaco T et al. Pharmaceuticals (Basel) 2018; 11 (4) Evaluation of Radiolabeled Girentuximab In Vitro and In Vivo.
Nagalakshmi VK et al. Clin Sci (Lond) 2018; 132 (23):2519-2545 Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Reactivity : Mouse
Masahiro Yasunaga et al. bioRxiv 2018; (Epub) Significant antitumour effect of an antibody against TMEM180, a new cancer-specific molecule.
Application : WB
Reactivity : Human
Xu G et al. Autophagy 2018;:43113 Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1 mRNA splicing.
Application : WB
Reactivity : Human
Sasa K et al. Sci Rep 2018; 8 (1):10579 Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.
Application : ICC/IF
Reactivity : Mouse
Fu JL et al. Hum Reprod 2018; (Epub) Suppression of COUP-TFII upregulates angiogenin and promotes angiogenesis in endometriosis.
Application : WB
Reactivity : Human
Hassan A et al. PLoS One 2018; 13 (5):e0197486 Acute intermittent hypoxia and rehabilitative training following cervical spinal injury alters neuronal hypoxia- and plasticity-associated protein expression.
Application : IHC-P
Reactivity : Rat
Yamamura H et al. Am J Physiol Cell Physiol 2018; (Epub) Hypoxic stress up-regulates Kir2.1 expression by a pathway including hypoxic-inducible factor-1 and dynamin2 in brain capillary endothelial cells.
Ho SY et al. BMC Cancer 2018; 18 (1):480 Development of a computational promoter with highly efficient expression in tumors.
Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Kuang Y et al. Cancer Med 2018; (Epub) Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis.
Application : WB
Reactivity : Human
Owada S et al. Oncol Rep 2018; 39 (4):1805-1812 Autophagy-mediated adaptation of hepatocellular carcinoma cells to hypoxia-mimicking conditions constitutes an attractive therapeutic target.
Kuriyama S et al. Cell Death Discovery 2018; (Epub) PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Application : WB
Reactivity : Human
Lee SH et al. J Tissue Eng Regen Med 2018; (Epub) Improvement of islet function and survival by integration of perfluorodecalin into microcapsules in vivo and in vitro.
Application : ICC/IF
Reactivity : Rat
Bin? L et al. Oncotarget 2017; 8 (48):83684-83697 Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.
Application : WB
Reactivity : Mouse
Sun W et al. Sci Rep 2017; 7 (1):7190 Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression.
Application : WB
Reactivity : Human
Shih JW et al. Nat Commun 2017; 8:15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Application : WB
Reactivity : Human
Kraus RJ et al. PLoS Pathog 2017; 13 (6):e1006404 Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter.
Application : ICC/IF
Reactivity : Mouse
Wang M et al. Theranostics 2017; 7 (5):1100-1113 AHNAK2 is a Novel Prognostic Marker and Oncogenic Protein for Clear Cell Renal Cell Carcinoma.
Kitajima S et al. Oncotarget 2017; 8 (70):114481-114494 Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells.
Application : WB
Reactivity : Human
Bunai K et al. J Cell Mol Med 2017; (Epub) TMEM207 hinders the tumour suppressor function of WWOX in oral squamous cell carcinoma.
Application : WB
Reactivity : Human
Li S et al. PLoS One 2017; 12 (11):e0187810 Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
Application : WB
Reactivity : Mouse
Ve?e?a1 J et al. Hindawi 2017; (Epub) Neural differentiation is inhibited through HIF1α/β-catenin signaling in embryoid bodies.
Application : WB
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10):e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Owada S et al. Anticancer Res 2017; 37 (9):4927-4934 An Adaptation System to Avoid Apoptosis via Autophagy Under Hypoxic Conditions in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Yan Q et al. Cancer Res 2017; (Epub) ANGPTL1 interacts with integrin α1β1 to suppress HCC angiogenesis and metastasis by inhibiting JAK2/STAT3 signaling.
Application : WB
Reactivity : Human
Xu M et al. Front. Pharmacol. 2017; (Epub) E-M, an Engineered Endostatin with High ATPase Activity, Inhibits the Recruitment and Alternative Activation of Macrophages in Non-small Cell Lung Cancer.
Application : WB
Reactivity : Human
Ji Y et al. Nat Commun 2017; 8:15308 Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation.
Application : WB
Reactivity : Human
Ku?era J et al. Hindawi 2017; (Epub) Hypoxia downregulates MAPK/ERK but not STAT3 signaling in ROS-dependent and HIF-1-independent manners in mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
Tsai TH et al. Molecules 2017; 22 (5) Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.
Application : WB
Reactivity : Human
Laubenthal L et al. J Dairy Sci 2017; (Epub) Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows.
Application : IHC-P
Reactivity : Bovine
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Fujiki K et al. J Biol Chem 2017; (Epub) Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Application : WB
Reactivity : Human
Nagamine A et al. JPS 2017; (Epub) The effects of DPP-4 inhibitor on hypoxia-induced apoptosis in human umbilical vein endothelial cells.
Application : ICC/IF
Reactivity : Human
Di K et al. Oncotarget 2016; 7 (47):77457-77467 Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
Application : WB
Reactivity : Human
Jou YC et al. Oncotarget 2016; 7 (40):65403-65417 Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation.
Application : WB
Reactivity : Human
Demetriades C et al. Nat Commun 2016; 7:10662 Lysosomal recruitment of TSC2 is a universal response to cellular stress.
Application : WB
Reactivity : Mouse
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Lin YC et al. Arterioscler Thromb Vasc Biol 2016; (Epub) Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.
Application : WB
Reactivity : Human
Barnes EA et al. FASEB J 2016; (Epub) Loss of smooth muscle cell hypoxia inducible factor-1α underlies increased vascular contractility in pulmonary hypertension.
Application : ICC/IF
Reactivity : Human
Chuang MH et al. Integr Cancer Ther 2016; (Epub) Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.
Application : IHC-P
Reactivity : Human
Wong MJ et al. Am J Physiol Lung Cell Mol Physiol 2016;:ajplung.00345.2016 Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling.
Chiang CH et al. Am J Cancer Res 2016; 6 (8):1785-98 MiR-182 promotes proliferation and invasion and elevates the HIF-1α-VEGF-A axis in breast cancer cells by targeting FBXW7.
Application : WB
Reactivity : Human
Kudova J et al. J Pineal Res 2016; Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization.
Application : WB
Reactivity : Mouse
Lin HC et al. Antioxid Redox Signal 2016; MicroRNA-183 mediates protective postconditioning of the liver by repressing Apaf-1.
Application : WB
Reactivity : Mouse
Logsdon DP et al. Mol Cancer Ther 2016; Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual-Targeting in Patient-Derived 3D Pancreatic Cancer Models.
Application : WB
Reactivity : Human
Lim NS et al. Wound Repair Regen 2016; (Epub) Combination of ciclopirox olamine and sphingosine-1-phosphate as granulation enhancer in diabetic wounds.
Hiromura M et al. Circ J 2016; (Epub) suppressive effects of glucose-dependent *otropic polypeptide on cardiac hypertrophy and fibrosis in angiotensin ii-infused mouse models.
Application : IHC-Fr
Reactivity : Mouse
Tsai SH et al. Sci Rep 2016; 6:28612 Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases.
Application : WB, ChIP assay
Reactivity : Human
Kudova J et al. PLoS One 2016; 11 (6):e0158358 HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
Application : WB
Reactivity : Mouse
Lin CS et al. Int J Mol Sci 2016; 17 (6) Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Application : WB
Reactivity : Human
Erapaneedi R et al. EMBO J 2016; 35 (1):102-13 A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level.
Application : IHC-Fr
Reactivity : Mouse
Jakubauskiene E et al. J Biol Chem 2015; 290 (29):18079-89 Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia.
Application : ChIP assay
Reactivity : Human
Matsuo T et al. Sci Rep 2015; 5:16842 Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.
Application : ICC/IF
Reactivity : Mouse
Kinoshita H et al. Carcinogenesis 2015; 36 (12):1511-20 Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments.
Application : WB
Reactivity : Human
Lucia Bino et al. Chemico-Biological Interactions 2015; The stabilization of hypoxia inducible factor modulates differentiation status and inhibits the proliferation of mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
Stanislava Slade?ek et al. 0 2015; Regulation of mitogen-activated protein kinases phosphatases under hypoxia
Application : WB
Reactivity : Human
Zhu Y et al. Pharmacol Res 2015; 93:36-42 Enhancement of vascular endothelial growth factor release in long-term drug-treated breast cancer via transient receptor potential channel 5-Ca(2+)-hypoxia-inducible factor 1α pathway.
Application : WB
Reactivity : Human
Chen PY et al. Food Funct 2015; 6 (1):286-95 Cytoprotective effects of fisetin against hypoxia-induced cell death in PC12 cells.
Application : WB
Reactivity : Rat
Chakravarthi BV et al. Oncotarget 2014; 5 (16):6654-69 The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.
Application : WB, ChIP assay
Reactivity : Human
Dahlmann M et al. Oncotarget 2014; 5 (10):3220-33 RAGE mediates S100A4-induced cell motility via MAPK/ERK and hypoxia signaling and is a prognostic biomarker for human colorectal cancer metastasis.
Application : WB
Reactivity : Human
Hou HH et al. Respir Res 2014; 15:106 PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.
Application : WB
Reactivity : Human
Morani F et al. J Mol Endocrinol 2014; 53 (2):247-58 PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells.
Pate KT et al. EMBO J 2014; Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.
Application : WB
Reactivity : Human
Chang LC et al. Br J Pharmacol 2014; YC-1 inhibits proliferation of breast cancer cells by downregulating EZH2 expression via activation of c-Cbl and ERK.
Application : WB
Reactivity : Human
Herrera I et al. J Biol Chem 2013; 288 (36):25964-75 Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption.
Application : WB, ICC/IF
Reactivity : Human
Misuno K et al. Stem Cell Res Ther 2013; 4 (6):156 Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.
Application : WB
Reactivity : Human
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