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HIF1 alpha antibody

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

Cat. No. GTX127309

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

Human, Mouse, Rat, Rabbit, Bovine
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a master regulator of homeostatic adaption to hypoxia (low oxygen supply) in various physiological and pathological conditions including normal development and cancer.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:570
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HepG2 (1% O2 treatment for 24hr) , 293T transfected HIF1A

Product Note

Knockdown/Knockout validation was supported by references (PMID:25428606)

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616089

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm , Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309

GTX127309 IHC-P Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody (GTX127309) dilution: 1:500.

Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309

GTX127309 ChIP assay Image

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309

GTX127309 IP Image

Immunoprecipitation of HIF1 alpha protein from HeLa whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309).
Western blot analysis was performed using HIF1 alpha antibody (GTX127309).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) Rat-2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28639619

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the 2022 in Sci Rep. PMID: 35027586

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the 2019 in Aging (Albany NY). PMID: 31584878

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35328751

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the 2019 in Int J Mol Sci. PMID: 30609861

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the 2019 in Int J Mol Sci. PMID: 30609861

Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309

GTX127309 IP Image

The data was published in the 2019 in Int J Mol Sci. PMID: 30609861

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the 2019 in Int J Mol Sci. PMID: 30609861

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27557492

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 30796225

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2018. PMID: 29721188

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27764809

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2017. PMID: 29383096

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Nat Commun in 2017.PMID: 28639619

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal PLoS One in 2017.PMID: 29121675

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28775317

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Nat Commun in 2016. PMID: 26868506

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28775317

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Cells in 2019. PMID: 31905895

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

The data was published in the journal PLoS Pathog in 2017. PMID: 28617871

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2017. PMID: 29383096

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27557492

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Oncotarget in 2017. PMID: 29137374

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Cancer Med in 2018.PMID: 29533007

Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309

GTX127309 IHC-P Image

The data was published in the journal Pharmaceuticals (Basel) in 2018.PMID: 30487460

Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309

GTX127309 IHC-P Image

The data was published in the 2022 in Open Life Sci. PMID: 35291563

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Stem Cell Res Ther in 2013. PMID: 24423398

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28775317

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27355368

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31174567

REFERENCE

REVIEW

HIF1 alpha antibody Cat. No. GTX127309

Rating ( Average 4.3 based on 26 users reviews)
Application
Western Blot(WB) ( Average 4.4 based on 20 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 3.8 based on 4 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 22-May-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC-3 and DU145 cells with/without drug C treatment
Amount used : 4.32 μg
Blocking : 5% BSA in PBST, 24°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 02-May-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW480 cells with/without 1% Hypoxia and/or HIF 1 alpha inhibitor treatment
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, RT°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 10-Feb-2022
Score :
Application Tested : IHC-P
Sample Species : Ms, Hu
Sample : Human and mouse liver tissue sections
Blocking : goat serum
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Citrate Buffer, 0.25Hr
Primary Antibody : 1:200, 4°C, 12Hr
Date : Anonymous submitted on 05-Dec-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human pancreatic cancer cell line
Amount used : 5μg
Blocking : 3% Skim milk/TBS-T, 24°C, 1Hr
Primary Antibody : 1:2000, 4°C, 20Hr
Date : Anonymous submitted on 30-Nov-2021
Score :
Application Tested : WB
Sample Species : Bov
Sample : Bovine brain capillary endothelial cells
Amount used : 40μg
Blocking : Skim milk, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Date : Anonymous submitted on 19-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco-2 cells
Amount used : 10 μg
Blocking : 5 % Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 27-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse fibroblast cell lyate
Blocking : 5% non-fat milk in TBST, 28°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Cytosol fraction of untreated cells
Lane 2: Cytosol fraction of CoCl2 treated cells (200 uM, 48 hr)
Lane 3: blank
Lane 4: Mito/Nucleus fraction of untreated cells
Lane 5: Mito/Nucleus fraction of CoCl2 treated cells (200 uM, 48 hr)
Date : Anonymous submitted on 25-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A375 cells
Blocking : 5% BSA in TBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 14-Sep-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : LoVo cells
Blocking : 3% skim-milk in TBST, 4°C, 18Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : LoVo cell were cultured using the hypoxic culture kit under hypoxic culture conditions (1% O2 at 37ºC) for 1, 2, 3, or 4 d. Lysates of normoxic and hypoxic cultured cells were separated on 8% SDS-PAGE gels and were immunoblotted using HIF-1 alpha Ab.
Date : Anonymous submitted on 14-Sep-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : DLD-1 cells
Blocking : 3% skim-milk in TBST, 4°C, 18Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : DLD-1 cell were cultured using the hypoxic culture kit under hypoxic culture conditions (1% O2 at 37ºC) for 1, 2, 3, or 4 d. Lysates of normoxic and hypoxic cultured cells were separated on 8% SDS-PAGE gels and were immunoblotted using HIF-1 alpha Ab.
Date : Anonymous submitted on 25-Apr-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : RAW264.7 cells
Amount used : 20 μg
Blocking : 3% non-fat dry milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Adult cardiac tissue
Blocking : 10% goat serum in PBS-TX100 0.1%, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton-X-100/Citrate Buffer pH6, 0.15Hr
Primary Antibody : 1:50, 4°C, 16Hr
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Renal clear cell carcinoma
Blocking : EnVISION FLEX PEROXIDASE-BLOCKING REAGENT (Dako, Ref. K800)
Fixation : 3.7% formalin
Permeabilization /Antigen retrieval : pH 9 Envision FLEX target retrieval solution, High pH, (Dako Ref. GV800)
Primary Antibody : 1:100, 25°C, 1Hr
Date : Anonymous submitted on 30-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10 μg
Blocking : ATTO EzBlock Chemi, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-P
Sample : HeLa xenograft
Blocking : 10% goat serum, 25°C, 0.08Hr
Fixation : Formalin Neutral Buffer
Permeabilization /Antigen retrieval : 0.2% Brij35 in PBS/Dako S2369
Primary Antibody : 1:100, 25°C, 0.6Hr
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Vehicle, Lane 2: 500 μM Cocl2 treatment for 4 hr
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Normoxia, Lane 2: 1% O2 treatment for 4 hr
Date : Anonymous submitted on 22-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : dental pulp stem cells
Amount used : 1x10^4 cells
Blocking : PVDF blocking reagent, 25°C, 0.25Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 02-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1:THP-1 cells (250ug/ml TPA treatment for 4h), Lane 2:THP-1 cells (250ug/ml TPA and 1 ug/ml LPS treatment for 4h)
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A: A549 cells, B: A549 cells (1mM DMOG treatment for 3.5 h)
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : brain tissue
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A:Human glioblastoma tissue, B:Human healthy brain tissue
Date : Anonymous submitted on 30-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : meniscal fibrochondrocytes
Amount used : 15μg
Blocking : BlockingPRO Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: meniscal fibrochondrocytes (normoxia), Lane 2: meniscal fibrochondrocytes (1% O2 treatment for 6hr)
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : endometriotic stromal cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane A:DMSO treatment (24hr) ; Lane B:0.1uM DFO treatment (24hr) ; Lane C:1uM DFO treatment (24hr)
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane A: HeLa cell lysate(normoxia), Lane B: HeLa cell lysate (500μM CoCl2 treatment)
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 37°C, 1Hr
Primary Antibody : 1:1000, 37°C, 1Hr
Lane Description : A: A431 whole cell lysate under normoxia, B: A431 whole cell lysate under hypoxia
Date : Anonymous submitted on 16-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 3% milk , 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Application Reference
Wu TH et al. Int J Mol Sci 2019; 20 (23)
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169