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HIF1 alpha antibody

KO/KD Comparison Orthogonal Overexpression
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309
Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

Cat No. GTX127309

Reference 99
16

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine
Package
100 μl ($329),
25 μl ($169)
See all HIF1 alpha products
PRODUCT

Summary

HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a master regulator of homeostatic adaption to hypoxia (low oxygen supply) in various physiological and pathological conditions including normal development and cancer.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:570
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

MCF-7 , HeLa(500uM CoCl for 24hr) , NIH-3T3 (500 μM CoCl2 treatment for 24 hr) , HepG2 whole cell lysate/extract (1% O2 treatment for 24hr) , 293T transfected HIF1A , HepG2 ( 200 μM CoCl2 treatment for 24 hr) , HepG2 (500 μM CoCl2 treatment for 24 hr)

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:25428606)

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm , Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis.
Samples: Untreated (left panel) and 500μM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min
Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000
Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Images captured by Olympus FV10i Confocal Laser Scanning Microscope

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

KO/KD

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309

GTX127309 IHC-P Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody (GTX127309) dilution: 1:500.
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309

GTX127309 IP Image

Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309).
Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309

GTX127309 ChIP assay Image

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B.30 μg HepG2 whole cell lysate/extract ( 200 μM CoCl2 treatment for 24 hr)
C. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr)
7.5% SDS-PAGE
HIF1 alpha antibody (GTX127309) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal Overexpression

The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The WB analysis of HIF1 alpha antibody was published by Misuno K and colleagues in the journal Stem Cell Res Ther in 2013.PMID: 24423398

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The WB analysis of HIF1 alpha antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 . PMID: 31174567

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The WB analysis of HIF1 alpha antibody was published by Kudová J and colleagues in the journal PLoS One in 2016 . PMID: 27355368

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

Orthogonal

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

The WB analysis of HIF1 alpha antibody was published by Demetriades C and colleagues in the journal Nat Commun in 2016.PMID: 26868506

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

The WB analysis of HIF1 alpha antibody was published by Sun W and colleagues in the journal Sci Rep in 2017.PMID: 28775317

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

KO/KD Orthogonal

The WB analysis of HIF1 alpha antibody was published by Tsai SH and colleagues in the journal Sci Rep in 2016.PMID: 27363580

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Eric Song et al. 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.
Application : IHC-Fr
Reactivity : Human
osef Ve?e?a et al. Stem Cell Res 2020; 45 : 101770 Hypoxia/Hif1α prevents premature neuronal differentiation of neural stem cells through the activation of Hes1.
Application : WB
Dou YQ et al. Theranostics 2020; 10 (3) : 1197-1212 Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia.
Application : WB, ICC/IF, ChIP assay
Reactivity : Human
Ve?e?a J et al. Stem Cell Res 2020; 45 : 101770 Hypoxia/Hif1α Prevents Premature Neuronal Differentiation of Neural Stem Cells Through the Activation of Hes1.
Application : WB
Reactivity : Mouse
Gojkovic M et al. Acta Physiol (Oxf) 2020; 229 (2) : e13461 Deregulated hypoxic response in myeloid cells: A model for high-altitude pulmonary oedema (HAPE).
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

HIF1 alpha antibody Cat No. GTX127309

Rating
( Average 4.2 based on 16 users reviews)
Application
Western Blot(WB)
( Average 4.4 based on 11 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 3.7 based on 3 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 25-Apr-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : RAW264.7 cells
Amount used : 20 μg
Blocking : 3% non-fat dry milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-HIF1 alpha antibody used in IHC (Frozen sections) (IHC-Fr). GTX127309
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Adult cardiac tissue
Blocking : 10% goat serum in PBS-TX100 0.1%, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton-X-100/Citrate Buffer pH6, 0.15Hr
Primary Antibody : 1:50, 4°C, 16Hr
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Renal clear cell carcinoma
Blocking : EnVISION FLEX PEROXIDASE-BLOCKING REAGENT (Dako, Ref. K800)
Fixation : 3.7% formalin
Permeabilization /Antigen retrieval : pH 9 Envision FLEX target retrieval solution, High pH, (Dako Ref. GV800)
Primary Antibody : 1:100, 25°C, 1Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10 μg
Blocking : ATTO EzBlock Chemi, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-P
Sample : HeLa xenograft
Blocking : 10% goat serum, 25°C, 0.08Hr
Fixation : Formalin Neutral Buffer
Permeabilization /Antigen retrieval : 0.2% Brij35 in PBS/Dako S2369
Primary Antibody : 1:100, 25°C, 0.6Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Vehicle, Lane 2: 500 μM Cocl2 treatment for 4 hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Normoxia, Lane 2: 1% O2 treatment for 4 hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 22-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : dental pulp stem cells
Amount used : 1x10^4 cells
Blocking : PVDF blocking reagent, 25°C, 0.25Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 02-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1:THP-1 cells (250ug/ml TPA treatment for 4h), Lane 2:THP-1 cells (250ug/ml TPA and 1 ug/ml LPS treatment for 4h)
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A: A549 cells, B: A549 cells (1mM DMOG treatment for 3.5 h)
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : brain tissue
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A:Human glioblastoma tissue, B:Human healthy brain tissue
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : meniscal fibrochondrocytes
Amount used : 15μg
Blocking : BlockingPRO Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: meniscal fibrochondrocytes (normoxia), Lane 2: meniscal fibrochondrocytes (1% O2 treatment for 6hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : endometriotic stromal cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane A：DMSO treatment (24hr) ; Lane B：0.1uM DFO treatment (24hr) ; Lane C：1uM DFO treatment (24hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane A: HeLa cell lysate(normoxia), Lane B: HeLa cell lysate (500μM CoCl2 treatment)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 37°C, 1Hr
Primary Antibody : 1:1000, 37°C, 1Hr
Lane Description : A: A431 whole cell lysate under normoxia, B: A431 whole cell lysate under hypoxia
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 16-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 3% milk , 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Eric Song et al. 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.
Application : IHC-Fr
Reactivity : Human
osef Ve?e?a et al. Stem Cell Res 2020; 45:101770 Hypoxia/Hif1α prevents premature neuronal differentiation of neural stem cells through the activation of Hes1.
Application : WB
Dou YQ et al. Theranostics 2020; 10 (3):1197-1212 Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia.
Application : WB, ICC/IF, ChIP assay
Reactivity : Human
Ve?e?a J et al. Stem Cell Res 2020; 45:101770 Hypoxia/Hif1α Prevents Premature Neuronal Differentiation of Neural Stem Cells Through the Activation of Hes1.
Application : WB
Reactivity : Mouse
Gojkovic M et al. Acta Physiol (Oxf) 2020; 229 (2):e13461 Deregulated hypoxic response in myeloid cells: A model for high-altitude pulmonary oedema (HAPE).
Application : WB
Reactivity : Mouse
Kato H et al. Oncogene. 2020; (Epub) Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization.
Application : WB
Reactivity : Human
Fujii M et al. Int Endod J 2020; (Epub) Hypoxia-inducible factor 1α promotes interleukin 1β and tumour necrosis factor α expression in lipopolysaccharide-stimulated human dental pulp cells.
Application : WB
Reactivity : Human
Zhang S et al. Gene 2020; 733:144383 MicroRNA-574-5p in gastric cancer cells promotes angiogenesis by targeting protein tyrosine phosphatase non-receptor type 3 (PTPN3).
Application : WB
Reactivity : Human
Chen W et al. Oncol Lett 2019; 17 (3):2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Manuprasert W et al. Asian Pac J Allergy Immunol 2019; (Epub) Intermittent hypoxia in rat enhancing peritoneal membrane thickening through HIF-1α-induced cytokines in peritoneum.
Reactivity : Rat
Chua KV et al. Cells 2019; 9 (1) Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90帢 Secretion, and Tumor Growth.
Application : WB
Reactivity : Human
Wu TH et al. Int J Mol Sci 2019; 20 (23) Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells.
Application : WB
Reactivity : Human
Pan CH et al. Aging (Albany NY) 2019; 11 (19):8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.
Application : WB
Reactivity : Rat
Zheng J et al. J Ethnopharmacol 2019;:112330 BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic mice.
Application : WB
Reactivity : Mouse
Ho YJ et al. Theranostics 2019; 9 (24):7370-7383 Normalization of Tumor Vasculature by Oxygen Microbubbles with Ultrasound.
Application : WB
Reactivity : Mouse
Li F et al. World J Pediatr 2019; The neuroprotective effect of mesenchymal stem cells is mediated through inhibition of apoptosis in hypoxic ischemic injury.
Application : WB
Reactivity : Human
Loh XY et al. Cancer Res 2019; RNA-binding protein ZFP36L1 suppresses hypoxia and cell cycle signaling.
Application : WB
Reactivity : Human
Tang K et al. Oncogene 2019; (Epub) Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.
Application : IP
Reactivity : Human
Kuo YL et al. Sci Rep 2019; 9 (1):10269 Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture.
Application : WB, ICC/IF, IHC-P
Reactivity : Human, Mouse
Lai PY et al. Physiol Genomics 2019; Adverse Early Life Environment Impairs Postnatal Lung Development in Mice.
Application : WB
Reactivity : Mouse
Offer S et al. J Exp Clin Cancer Res 2019; 38 (1):241 Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration.
Application : WB
Reactivity : Human
Holzer T et al. J Cell Biol 2019; 218 (6):1853-1870 Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases.
Application : WB
Reactivity : Mouse
Ma Z et al. Cell Cycle 2019; 18 (13):1473-1489 Deletion of clock gene Bmal1 impaired the chondrocyte function due to disruption of the HIF1帢-VEGF signaling pathway.
Application : IHC-P
Reactivity : Mouse
Zhou M et al. Sci Rep 2019; 9 (1):6035 The pro-angiogenic role of hypoxia inducible factor stabilizer * and its application in an in vivo tissue engineering chamber model.
Application : IHC-P
Reactivity : Human
Wang X et al. J Steroid Biochem Mol Biol 2019; 191:105370 Transcription regulation of NRF1 on StAR reduces testosterone synthesis in hypoxemic murine.
Application : WB
Reactivity : Mouse
Song S et al. Am J Transl Res 2019; 11 (4):2155-2167 Blocking transforming growth factor-beta reduces the migration and invasion of the residual tumour after TAE.
Application : IHC-Fr
Reactivity : Rabbit
Ezzeddini R et al. Life Sci 2019; 224:169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.
Application : WB
Reactivity : Human
Kurelac I et al. Nat Commun 2019; 10 (1):903 Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.
Application : WB
Reactivity : Human
Wenli Chen et al. Oncology Letters 2019;:2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Application : WB, IHC-P
Yasunaga M et al. Cancer Sci 2019; 110 (2):761-770 Significant antitumor effect of an antibody against TMEM180, a new colorectal cancer-specific molecule.
Application : WB
Reactivity : Human
Hsu HL et al. Int J Mol Sci 2019; 20 (1) Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.
Application : WB
Reactivity : Human
Kitajima S et al. Oncotarget 2018; 9 (27):19123-19135 Hypoxia-inducible factor-2 alpha up-regulates CD70 under hypoxia and enhances anchorage-independent growth and aggressiveness in cancer cells.
Basaco T et al. Pharmaceuticals (Basel) 2018; 11 (4) Evaluation of Radiolabeled Girentuximab In Vitro and In Vivo.
Nagalakshmi VK et al. Clin Sci (Lond) 2018; 132 (23):2519-2545 Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Application : IHC-Fr
Reactivity : Mouse
Masahiro Yasunaga et al. bioRxiv 2018; (Epub) Significant antitumour effect of an antibody against TMEM180, a new cancer-specific molecule.
Application : WB
Reactivity : Human
Xu G et al. Autophagy 2018;:43113 Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1 mRNA splicing.
Application : WB
Reactivity : Human
Sasa K et al. Sci Rep 2018; 8 (1):10579 Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.
Application : ICC/IF
Reactivity : Mouse
Fu JL et al. Hum Reprod 2018; (Epub) Suppression of COUP-TFII upregulates angiogenin and promotes angiogenesis in endometriosis.
Application : WB
Reactivity : Human
Hassan A et al. PLoS One 2018; 13 (5):e0197486 Acute intermittent hypoxia and rehabilitative training following cervical spinal injury alters neuronal hypoxia- and plasticity-associated protein expression.
Application : IHC-P
Reactivity : Rat
Yamamura H et al. Am J Physiol Cell Physiol 2018; (Epub) Hypoxic stress up-regulates Kir2.1 expression by a pathway including hypoxic-inducible factor-1 and dynamin2 in brain capillary endothelial cells.
Ho SY et al. BMC Cancer 2018; 18 (1):480 Development of a computational promoter with highly efficient expression in tumors.
Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Kuang Y et al. Cancer Med 2018; (Epub) Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis.
Application : WB
Reactivity : Human
Owada S et al. Oncol Rep 2018; 39 (4):1805-1812 Autophagy-mediated adaptation of hepatocellular carcinoma cells to hypoxia-mimicking conditions constitutes an attractive therapeutic target.
Kuriyama S et al. Cell Death Discovery 2018; (Epub) PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Application : WB
Reactivity : Human
Lee SH et al. J Tissue Eng Regen Med 2018; (Epub) Improvement of islet function and survival by integration of perfluorodecalin into microcapsules in vivo and in vitro.
Application : ICC/IF
Reactivity : Rat
Bin? L et al. Oncotarget 2017; 8 (48):83684-83697 Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.
Application : WB
Reactivity : Mouse
Sun W et al. Sci Rep 2017; 7 (1):7190 Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression.
Application : WB
Reactivity : Human
Shih JW et al. Nat Commun 2017; 8:15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Application : WB
Reactivity : Human
Kraus RJ et al. PLoS Pathog 2017; 13 (6):e1006404 Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter.
Application : ICC/IF
Reactivity : Mouse
Wang M et al. Theranostics 2017; 7 (5):1100-1113 AHNAK2 is a Novel Prognostic Marker and Oncogenic Protein for Clear Cell Renal Cell Carcinoma.
Kitajima S et al. Oncotarget 2017; 8 (70):114481-114494 Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells.
Application : WB
Reactivity : Human
Bunai K et al. J Cell Mol Med 2017; (Epub) TMEM207 hinders the tumour suppressor function of WWOX in oral squamous cell carcinoma.
Application : WB
Reactivity : Human
Li S et al. PLoS One 2017; 12 (11):e0187810 Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
Application : WB
Reactivity : Mouse
Ve?e?a1 J et al. Hindawi 2017; (Epub) Neural differentiation is inhibited through HIF1α/β-catenin signaling in embryoid bodies.
Application : WB
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10):e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Owada S et al. Anticancer Res 2017; 37 (9):4927-4934 An Adaptation System to Avoid Apoptosis via Autophagy Under Hypoxic Conditions in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Yan Q et al. Cancer Res 2017; (Epub) ANGPTL1 interacts with integrin α1β1 to suppress HCC angiogenesis and metastasis by inhibiting JAK2/STAT3 signaling.
Application : WB
Reactivity : Human
Xu M et al. Front. Pharmacol. 2017; (Epub) E-M, an Engineered Endostatin with High ATPase Activity, Inhibits the Recruitment and Alternative Activation of Macrophages in Non-small Cell Lung Cancer.
Application : WB
Reactivity : Human
Ji Y et al. Nat Commun 2017; 8:15308 Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation.
Application : WB
Reactivity : Human
Ku?era J et al. Hindawi 2017; (Epub) Hypoxia downregulates MAPK/ERK but not STAT3 signaling in ROS-dependent and HIF-1-independent manners in mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
Tsai TH et al. Molecules 2017; 22 (5) Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.
Application : WB
Reactivity : Human
Laubenthal L et al. J Dairy Sci 2017; (Epub) Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows.
Application : IHC-P
Reactivity : Bovine
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Fujiki K et al. J Biol Chem 2017; (Epub) Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Application : WB
Reactivity : Human
Nagamine A et al. JPS 2017; (Epub) The effects of DPP-4 inhibitor on hypoxia-induced apoptosis in human umbilical vein endothelial cells.
Application : ICC/IF
Reactivity : Human
Di K et al. Oncotarget 2016; 7 (47):77457-77467 Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
Application : WB
Reactivity : Human
Jou YC et al. Oncotarget 2016; 7 (40):65403-65417 Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation.
Application : WB
Reactivity : Human
Demetriades C et al. Nat Commun 2016; 7:10662 Lysosomal recruitment of TSC2 is a universal response to cellular stress.
Application : WB
Reactivity : Mouse
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Lin YC et al. Arterioscler Thromb Vasc Biol 2016; (Epub) Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.
Application : WB
Reactivity : Human
Barnes EA et al. FASEB J 2016; (Epub) Loss of smooth muscle cell hypoxia inducible factor-1α underlies increased vascular contractility in pulmonary hypertension.
Application : ICC/IF
Reactivity : Human
Chuang MH et al. Integr Cancer Ther 2016; (Epub) Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.
Application : IHC-P
Reactivity : Human
Wong MJ et al. Am J Physiol Lung Cell Mol Physiol 2016;:ajplung.00345.2016 Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling.
Chiang CH et al. Am J Cancer Res 2016; 6 (8):1785-98 MiR-182 promotes proliferation and invasion and elevates the HIF-1α-VEGF-A axis in breast cancer cells by targeting FBXW7.
Application : WB
Reactivity : Human
Kudova J et al. J Pineal Res 2016; Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization.
Application : WB
Reactivity : Mouse
Lin HC et al. Antioxid Redox Signal 2016; MicroRNA-183 mediates protective postconditioning of the liver by repressing Apaf-1.
Application : WB
Reactivity : Mouse
Logsdon DP et al. Mol Cancer Ther 2016; Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual-Targeting in Patient-Derived 3D Pancreatic Cancer Models.
Application : WB
Reactivity : Human
Lim NS et al. Wound Repair Regen 2016; (Epub) Combination of ciclopirox olamine and sphingosine-1-phosphate as granulation enhancer in diabetic wounds.
Hiromura M et al. Circ J 2016; (Epub) suppressive effects of glucose-dependent *otropic polypeptide on cardiac hypertrophy and fibrosis in angiotensin ii-infused mouse models.
Application : IHC-Fr
Reactivity : Mouse
Tsai SH et al. Sci Rep 2016; 6:28612 Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases.
Application : WB, ChIP assay
Reactivity : Human
Kudova J et al. PLoS One 2016; 11 (6):e0158358 HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
Application : WB
Reactivity : Mouse
Lin CS et al. Int J Mol Sci 2016; 17 (6) Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Application : WB
Reactivity : Human
Erapaneedi R et al. EMBO J 2016; 35 (1):102-13 A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level.
Application : IHC-Fr
Reactivity : Mouse
Jakubauskiene E et al. J Biol Chem 2015; 290 (29):18079-89 Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia.
Application : ChIP assay
Reactivity : Human
Matsuo T et al. Sci Rep 2015; 5:16842 Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.
Application : ICC/IF
Reactivity : Mouse
Kinoshita H et al. Carcinogenesis 2015; 36 (12):1511-20 Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments.
Application : WB
Reactivity : Human
Lucia Bino et al. Chemico-Biological Interactions 2015; The stabilization of hypoxia inducible factor modulates differentiation status and inhibits the proliferation of mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
Stanislava Slade?ek et al. 0 2015; Regulation of mitogen-activated protein kinases phosphatases under hypoxia
Application : WB
Reactivity : Human
Zhu Y et al. Pharmacol Res 2015; 93:36-42 Enhancement of vascular endothelial growth factor release in long-term drug-treated breast cancer via transient receptor potential channel 5-Ca(2+)-hypoxia-inducible factor 1α pathway.
Application : WB
Reactivity : Human
Chen PY et al. Food Funct 2015; 6 (1):286-95 Cytoprotective effects of fisetin against hypoxia-induced cell death in PC12 cells.
Application : WB
Reactivity : Rat
Chakravarthi BV et al. Oncotarget 2014; 5 (16):6654-69 The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.
Application : WB, ChIP assay
Reactivity : Human
Dahlmann M et al. Oncotarget 2014; 5 (10):3220-33 RAGE mediates S100A4-induced cell motility via MAPK/ERK and hypoxia signaling and is a prognostic biomarker for human colorectal cancer metastasis.
Application : WB
Reactivity : Human
Hou HH et al. Respir Res 2014; 15:106 PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.
Application : WB
Reactivity : Human
Morani F et al. J Mol Endocrinol 2014; 53 (2):247-58 PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells.
Pate KT et al. EMBO J 2014; Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.
Application : WB
Reactivity : Human
Chang LC et al. Br J Pharmacol 2014; YC-1 inhibits proliferation of breast cancer cells by downregulating EZH2 expression via activation of c-Cbl and ERK.
Application : WB
Reactivity : Human
Herrera I et al. J Biol Chem 2013; 288 (36):25964-75 Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption.
Application : WB, ICC/IF
Reactivity : Human
Misuno K et al. Stem Cell Res Ther 2013; 4 (6):156 Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.
Application : WB
Reactivity : Human
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