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HIF1 alpha antibody

KO/KD Comparison Orthogonal Overexpression
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309
Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

Cat No. GTX127309

Reference 140
54

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine
Package
100 μl ($329),
25 μl ($169)
See all HIF1 alpha products
PRODUCT

Summary

HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a master regulator of homeostatic adaption to hypoxia (low oxygen supply) in various physiological and pathological conditions including normal development and cancer.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:570
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

MCF-7 , HeLa(500uM CoCl for 24hr) , NIH-3T3 (500 μM CoCl2 treatment for 24 hr) , HepG2 whole cell lysate/extract (1% O2 treatment for 24hr) , 293T transfected HIF1A , HepG2 ( 200 μM CoCl2 treatment for 24 hr) , HepG2 (500 μM CoCl2 treatment for 24 hr)

Product Note

Knockdown/Knockout validation was supported by references (PMID:25428606)

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616089

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

Cellular Localization

Cytoplasm , Nucleus

Background

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis.
Samples: Untreated (left panel) and 500μM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min
Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000
Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Images captured by Olympus FV10i Confocal Laser Scanning Microscope

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

KO/KD

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309

GTX127309 IHC-P Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody (GTX127309) dilution: 1:500.
Anti-HIF1 alpha antibody used in Immunoprecipitation (IP). GTX127309

GTX127309 IP Image

Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309).
Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HIF1 alpha antibody used in ChIP assay (ChIP assay). GTX127309

GTX127309 ChIP assay Image

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) Rat-2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309

GTX127309 ICC/IF Image

Orthogonal

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309

GTX127309 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

YF Yang et al. et al. J Cell Mol Med 2022; UBE2C triggers HIF-1α-glycolytic flux in head and neck squamous cell carcinoma
Application : WB
Reactivity : Human
Giovannucci TA et al. Autophagy 2021; : 1-17 Identification of a novel compound that simultaneously impairs the ubiquitin-proteasome system and autophagy.
Application : WB
Reactivity : Human
Shih HJ et al. Sci Rep 2021; 11 (1) : 22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Application : WB, ICC/IF
Reactivity : Human
Owada S et al. Anticancer Res 2021; 41 (12) : 6051-6059 Evaluation of Erastin as a Therapeutic Agent Under Hypoxic Conditions in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Peng G et al. J Exp Clin Cancer Res 2021; 40 (1) : 278 The HIF1α-PDGFD-PDGFRα axis controls glioblastoma growth at normoxia/mild-hypoxia and confers sensitivity to targeted therapy by echinomycin.
Application : WB
Reactivity : Human
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REVIEW

HIF1 alpha antibody Cat No. GTX127309

Rating
( Average 4.6 based on 54 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 46 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 5 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 10-Feb-2022
Score :
Application Tested : IHC-P
Sample Species : Ms, Hu
Sample : Human and mouse liver tissue sections
Blocking : goat serum
Fixation : 4% PFA
Permeabilization /Antigen retrieval : Citrate Buffer, 0.25Hr
Primary Antibody : 1:200, 4°C, 12Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Dec-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human pancreatic cancer cell line
Amount used : 5μg
Blocking : 3% Skim milk/TBS-T, 24°C, 1Hr
Primary Antibody : 1:2000, 4°C, 20Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Nov-2021
Score :
Application Tested : WB
Sample Species : Bov
Sample : Bovine brain capillary endothelial cells
Amount used : 40μg
Blocking : Skim milk, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 28169297
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018. PMID: 29118119
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018. PMID: 30209172
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018. PMID: 30209172
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018. PMID: 30209172
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018. PMID: 30209172
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 19-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco-2 cells
Amount used : 10 μg
Blocking : 5 % Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 27-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse fibroblast cell lyate
Blocking : 5% non-fat milk in TBST, 28°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Cytosol fraction of untreated cells
Lane 2: Cytosol fraction of CoCl2 treated cells (200 uM, 48 hr)
Lane 3: blank
Lane 4: Mito/Nucleus fraction of untreated cells
Lane 5: Mito/Nucleus fraction of CoCl2 treated cells (200 uM, 48 hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 25-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A375 cells
Blocking : 5% BSA in TBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27355368
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2013. PMID: 24423398
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28775317
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Exp Clin Cancer Res in 2019. PMID: 31174567
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016. PMID: 26868506
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28775317
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28775317
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27363580
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cells in 2019. PMID: 31905895
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30796225
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29721188
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29383096
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27764809
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27557492
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29383096
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 27557492
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28617871
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 29137374
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Sep-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : LoVo cells
Blocking : 3% skim-milk in TBST, 4°C, 18Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : LoVo cell were cultured using the hypoxic culture kit under hypoxic culture conditions (1% O2 at 37ºC) for 1, 2, 3, or 4 d. Lysates of normoxic and hypoxic cultured cells were separated on 8% SDS-PAGE gels and were immunoblotted using HIF-1 alpha Ab.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Sep-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : DLD-1 cells
Blocking : 3% skim-milk in TBST, 4°C, 18Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : DLD-1 cell were cultured using the hypoxic culture kit under hypoxic culture conditions (1% O2 at 37ºC) for 1, 2, 3, or 4 d. Lysates of normoxic and hypoxic cultured cells were separated on 8% SDS-PAGE gels and were immunoblotted using HIF-1 alpha Ab.
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 25-Apr-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : RAW264.7 cells
Amount used : 20 μg
Blocking : 3% non-fat dry milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-HIF1 alpha antibody used in IHC (Frozen sections) (IHC-Fr). GTX127309
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Adult cardiac tissue
Blocking : 10% goat serum in PBS-TX100 0.1%, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton-X-100/Citrate Buffer pH6, 0.15Hr
Primary Antibody : 1:50, 4°C, 16Hr
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 14-Feb-2019
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Renal clear cell carcinoma
Blocking : EnVISION FLEX PEROXIDASE-BLOCKING REAGENT (Dako, Ref. K800)
Fixation : 3.7% formalin
Permeabilization /Antigen retrieval : pH 9 Envision FLEX target retrieval solution, High pH, (Dako Ref. GV800)
Primary Antibody : 1:100, 25°C, 1Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10 μg
Blocking : ATTO EzBlock Chemi, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-P
Sample : HeLa xenograft
Blocking : 10% goat serum, 25°C, 0.08Hr
Fixation : Formalin Neutral Buffer
Permeabilization /Antigen retrieval : 0.2% Brij35 in PBS/Dako S2369
Primary Antibody : 1:100, 25°C, 0.6Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Vehicle, Lane 2: 500 μM Cocl2 treatment for 4 hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 05-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1: Normoxia, Lane 2: 1% O2 treatment for 4 hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 22-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : dental pulp stem cells
Amount used : 1x10^4 cells
Blocking : PVDF blocking reagent, 25°C, 0.25Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 02-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane 1:THP-1 cells (250ug/ml TPA treatment for 4h), Lane 2:THP-1 cells (250ug/ml TPA and 1 ug/ml LPS treatment for 4h)
Anti-HIF1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127309
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A: A549 cells, B: A549 cells (1mM DMOG treatment for 3.5 h)
Anti-HIF1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX127309
Date : Anonymous submitted on 29-Jan-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : brain tissue
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 95°C, 20minHr
Primary Antibody : 1:1000, 25°C, 1Hr
Figure Note : A:Human glioblastoma tissue, B:Human healthy brain tissue
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 30-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : meniscal fibrochondrocytes
Amount used : 15μg
Blocking : BlockingPRO Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: meniscal fibrochondrocytes (normoxia), Lane 2: meniscal fibrochondrocytes (1% O2 treatment for 6hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 08-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : endometriotic stromal cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Lane Description : Lane A：DMSO treatment (24hr) ; Lane B：0.1uM DFO treatment (24hr) ; Lane C：1uM DFO treatment (24hr)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane A: HeLa cell lysate(normoxia), Lane B: HeLa cell lysate (500μM CoCl2 treatment)
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 27-Aug-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : A431 cells
Amount used : 20μg
Blocking : 5% milk in TBST, 37°C, 1Hr
Primary Antibody : 1:1000, 37°C, 1Hr
Lane Description : A: A431 whole cell lysate under normoxia, B: A431 whole cell lysate under hypoxia
Anti-HIF1 alpha antibody used in Western Blot (WB). GTX127309
Date : Anonymous submitted on 16-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 40μg
Blocking : 3% milk , 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
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Application Reference
YF Yang et al. et al. J Cell Mol Med 2022; UBE2C triggers HIF-1α-glycolytic flux in head and neck squamous cell carcinoma
Application : WB
Reactivity : Human
Giovannucci TA et al. Autophagy 2021;:1-17 Identification of a novel compound that simultaneously impairs the ubiquitin-proteasome system and autophagy.
Application : WB
Reactivity : Human
Shih HJ et al. Sci Rep 2021; 11 (1):22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
Application : WB, ICC/IF
Reactivity : Human
Owada S et al. Anticancer Res 2021; 41 (12):6051-6059 Evaluation of Erastin as a Therapeutic Agent Under Hypoxic Conditions in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Peng G et al. J Exp Clin Cancer Res 2021; 40 (1):278 The HIF1α-PDGFD-PDGFRα axis controls glioblastoma growth at normoxia/mild-hypoxia and confers sensitivity to targeted therapy by echinomycin.
Application : WB
Reactivity : Human
Chen YC et al. Cell Biol Toxicol 2021; (Epub) Vitamin D3 decreases TNF-α-induced inflammation in lung epithelial cells through a reduction in mitochondrial fission and mitophagy.
Application : WB
Reactivity : Human
N?chel J et al. Mol Cell 2021; 81 (16):3275-3293.e12 An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.
Takenaga K et al. Sci Rep 2021; 11 (1):13302 MCT4 is induced by metastasis-enhancing pathogenic mitochondrial NADH dehydrogenase gene mutations and can be a therapeutic target.
Application : WB
Reactivity : Mouse
Hsu CG et al. J Immunol 2021; Phosphodiesterase 10A Is a Key Mediator of Lung Inflammation.
Sun X et al. Front Genet 2021; 12:680928 Expression and Clinical Significance of HKII and HIF-1α in Grade Groups of Prostate Cancer.
Application : IHC-P
Reactivity : Human
Coronel-Hern?ndez J et al. Front Oncol 2021; 11:594200 Combination of Metformin, Sodium Oxamate and Doxorubicin Induces Apoptosis and Autophagy in Colorectal Cancer Cells via Downregulation HIF-1α.
Application : WB
Gampala S et al. Br J Cancer 2021; 124 (9):1566-1580 Exploring transcriptional regulators Ref-1 and STAT3 as therapeutic targets in malignant peripheral nerve sheath tumours.
Application : WB
Reactivity : Human
Nadeau JR et al. Exp Neurol 2021; 341:113671 Acute intermittent hypoxia enhances regeneration of surgically repaired peripheral nerves in a manner akin to electrical stimulation.
Ezzeddini R et al. J Physiol Biochem 2021; 77 (2):249-260 Downregulation of fatty acid oxidation by involvement of HIF-1α and PPARγ in human gastric adenocarcinoma and related clinical significance.
Application : WB
Nakashima M et al. Cancer Sci 2021; 112 (6):2542-2555 Differentiation of Hodgkin lymphoma cells by reactive oxygen species and regulation by heme oxygenase-1 through HIF-1α.
Application : ICC/IF
Reactivity : Human
Kitajima S et al. Sci Rep 2021; 11 (1):4538 A KDM6 inhibitor potently induces ATF4 and its target gene expression through HRI activation and by UTX inhibition.
Application : WB
Reactivity : Human
Nishida D et al. Sci Rep 2021; 11 (1):4575 RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp.
Weng M et al. Front Cell Dev Biol 2021; 9:642930 Hypoxia-Induced LIN28A mRNA Promotes the Metastasis of Colon Cancer in a Protein-Coding-Independent Manner.
Application : WB, IHC-P
Reactivity : Human
Douiev L et al. Cells 2021; 10 (2) Upregulation of COX4-2 via HIF-1α in Mitochondrial COX4-1 Deficiency.
Application : ICC/IF
Reactivity : Human
Sasaki Y et al. Cancer Sci 2021; 112 (3):1251-1261 Expression of asporin reprograms cancer cells to acquire resistance to oxidative stress.
Application : WB
Reactivity : Human
Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Application : IHC-Fr
Reactivity : Mouse
Song E et al. bioRxiv 2020; (Epub) Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Ye D et al. Stem Cell Res Ther 2020; 11 (1):382 Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus.
Chen KC et al. Int J Mol Sci 2020; 21 (9) Bicalutamide Elicits Renal Damage by Causing Mitochondrial Dysfunction via ROS Damage and Upregulation of HIF-1.
Arellano-Buend?a AS et al. Antioxidants (Basel) 2020; 9 (11) Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes.
Application : WB
Reactivity : Rat
Cirotti C et al. EMBO Rep 2020;:e50500 Redox activation of ATM enhances GSNOR translation to sustain mitophagy and tolerance to oxidative stress.
Application : WB
Reactivity : Human
Ueshima E et al. Liver Cancer 2020; 9 (1):63-72 Hepatic Artery Embolization Induces the Local Overexpression of Transforming Growth Factor β1 in a Rat Hepatoma Model.
Chen HY et al. Chem Biol Interact 2020; 331:109249 TGF-β1 signaling protects retinal ganglion cells from oxidative stress via modulation of the HO-1/Nrf2 pathway.
Application : WB
Reactivity : Mouse
Hou J et al. Nat Cell Biol 2020; 22 (10):1264-1275 PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis.
Application : WB
Reactivity : Human
Tazawa K et al. Am J Pathol 2020; 190 (12):2417-2426 Transient Receptor Potential Ankyrin 1 Is Up-Regulated in Response to Lipopolysaccharide via P38/Mitogen-Activated Protein Kinase in Dental Pulp Cells and Promotes Mineralization.
Application : WB
Reactivity : Human
Owada S et al. Anticancer Res 2020; 40 (9):5071-5079 Setanaxib as a Potent Hypoxia-specific Therapeutic Agent Against Liver Cancer.
Application : WB
Reactivity : Human
Zhao Y et al. Am J Physiol Renal Physiol 2020; 319 (2):F345-F357 Endothelial prolyl hydroxylase 2 is necessary for angiotensin II-mediated renal fibrosis and injury.
Application : WB
Reactivity : Mouse
Bailey CM et al. Nanomedicine 2020; 29:102278 Liposomal formulation of HIF-1α inhibitor echinomycin eliminates established metastases of triple-negative breast cancer.
Application : WB
Reactivity : Human
Chan CH et al. Aging (Albany NY) 2020; 12 (18):17930-17947 Modulating redox homeostasis and cellular reprogramming through inhibited methylenetetrahydrofolate dehydrogenase 2 enzymatic activities in lung cancer.
Application : WB
Reactivity : Human
Takaki H et al. J Vasc Interv Radiol 2020; 31 (9):1475-1482.e2 Hepatic Artery Embolization Enhances Expression of Programmed Cell Death 1 Ligand 1 in an Orthotopic Rat Hepatocellular Carcinoma Model: In?Vivo and in?Vitro Experimentation.
Application : WB, IHC-P
Reactivity : Rat
Mochizuki M et al. Stem Cell Res Ther 2020; 11 (1):267 Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture.
Application : ICC/IF
Reactivity : Human
Eric Song et al. 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.
Application : IHC-Fr
Reactivity : Human
Dou YQ et al. Theranostics 2020; 10 (3):1197-1212 Smooth muscle SIRT1 reprograms endothelial cells to suppress angiogenesis after ischemia.
Application : WB, ICC/IF, ChIP assay
Reactivity : Human
Ve?e?a J et al. Stem Cell Res 2020; 45:101770 Hypoxia/Hif1α Prevents Premature Neuronal Differentiation of Neural Stem Cells Through the Activation of Hes1.
Application : WB
Reactivity : Mouse
Gojkovic M et al. Acta Physiol (Oxf) 2020; 229 (2):e13461 Deregulated hypoxic response in myeloid cells: A model for high-altitude pulmonary oedema (HAPE).
Application : WB
Reactivity : Mouse
Kato H et al. Oncogene. 2020; (Epub) Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization.
Application : WB
Reactivity : Human
Fujii M et al. Int Endod J 2020; (Epub) Hypoxia-inducible factor 1α promotes interleukin 1β and tumour necrosis factor α expression in lipopolysaccharide-stimulated human dental pulp cells.
Application : WB
Reactivity : Human
Zhang S et al. Gene 2020; 733:144383 MicroRNA-574-5p in gastric cancer cells promotes angiogenesis by targeting protein tyrosine phosphatase non-receptor type 3 (PTPN3).
Application : WB
Reactivity : Human
Singh M et al. Cancer Res 2019; 79 (11):2962-2977 Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration.
Chen W et al. Oncol Lett 2019; 17 (3):2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Manuprasert W et al. Asian Pac J Allergy Immunol 2019; (Epub) Intermittent hypoxia in rat enhancing peritoneal membrane thickening through HIF-1α-induced cytokines in peritoneum.
Reactivity : Rat
Chua KV et al. Cells 2019; 9 (1) Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90帢 Secretion, and Tumor Growth.
Application : WB
Reactivity : Human
Wu TH et al. Int J Mol Sci 2019; 20 (23) Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells.
Application : WB
Reactivity : Human
Pan CH et al. Aging (Albany NY) 2019; 11 (19):8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.
Application : WB
Reactivity : Rat
Zheng J et al. J Ethnopharmacol 2019;:112330 BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic mice.
Application : WB
Reactivity : Mouse
Ho YJ et al. Theranostics 2019; 9 (24):7370-7383 Normalization of Tumor Vasculature by Oxygen Microbubbles with Ultrasound.
Application : WB
Reactivity : Mouse
Li F et al. World J Pediatr 2019; The neuroprotective effect of mesenchymal stem cells is mediated through inhibition of apoptosis in hypoxic ischemic injury.
Application : WB
Reactivity : Human
Loh XY et al. Cancer Res 2019; RNA-binding protein ZFP36L1 suppresses hypoxia and cell cycle signaling.
Application : WB
Reactivity : Human
Tang K et al. Oncogene 2019; (Epub) Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.
Application : IP
Reactivity : Human
Kuo YL et al. Sci Rep 2019; 9 (1):10269 Hypoxia-induced epithelial-mesenchymal transition and fibrosis for the development of breast capsular contracture.
Application : WB, ICC/IF, IHC-P
Reactivity : Human, Mouse
Lai PY et al. Physiol Genomics 2019; Adverse Early Life Environment Impairs Postnatal Lung Development in Mice.
Application : WB
Reactivity : Mouse
Offer S et al. J Exp Clin Cancer Res 2019; 38 (1):241 Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration.
Application : WB
Reactivity : Human
Holzer T et al. J Cell Biol 2019; 218 (6):1853-1870 Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases.
Application : WB
Reactivity : Mouse
Ma Z et al. Cell Cycle 2019; 18 (13):1473-1489 Deletion of clock gene Bmal1 impaired the chondrocyte function due to disruption of the HIF1帢-VEGF signaling pathway.
Application : IHC-P
Reactivity : Mouse
Zhou M et al. Sci Rep 2019; 9 (1):6035 The pro-angiogenic role of hypoxia inducible factor stabilizer * and its application in an in vivo tissue engineering chamber model.
Application : IHC-P
Reactivity : Human
Wang X et al. J Steroid Biochem Mol Biol 2019; 191:105370 Transcription regulation of NRF1 on StAR reduces testosterone synthesis in hypoxemic murine.
Application : WB
Reactivity : Mouse
Song S et al. Am J Transl Res 2019; 11 (4):2155-2167 Blocking transforming growth factor-beta reduces the migration and invasion of the residual tumour after TAE.
Application : IHC-Fr
Reactivity : Rabbit
Ezzeddini R et al. Life Sci 2019; 224:169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.
Application : WB
Reactivity : Human
Kurelac I et al. Nat Commun 2019; 10 (1):903 Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.
Application : WB
Reactivity : Human
Wenli Chen et al. Oncology Letters 2019;:2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Application : WB, IHC-P
Yasunaga M et al. Cancer Sci 2019; 110 (2):761-770 Significant antitumor effect of an antibody against TMEM180, a new colorectal cancer-specific molecule.
Application : WB
Reactivity : Human
Hsu HL et al. Int J Mol Sci 2019; 20 (1) Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.
Application : WB
Reactivity : Human
Cha Yang et al. J Virol 2018; 0 (0) The Nucleolar Protein LYAR Facilitates Ribonucleoprotein Assembly of Influenza A Virus.
Application : WB, IHC-P
Reactivity : Human
Judith Oymans et al. J Virol 2018; 92 (3) A Mechanism for Priming and Realignment during Influenza A Virus Replication.
Application : WB
Reactivity : Human
Lai HH et al. J Clin Invest 2018; 128 (2):625-643 HIF-1α promotes autophagic proteolysis of Dicer and enhances tumor metastasis.
Kuriyama S et al. Cell Death Discov 2018; 4:11 PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Kitajima S et al. Oncotarget 2018; 9 (27):19123-19135 Hypoxia-inducible factor-2 alpha up-regulates CD70 under hypoxia and enhances anchorage-independent growth and aggressiveness in cancer cells.
Basaco T et al. Pharmaceuticals (Basel) 2018; 11 (4) Evaluation of Radiolabeled Girentuximab In Vitro and In Vivo.
Nagalakshmi VK et al. Clin Sci (Lond) 2018; 132 (23):2519-2545 Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Application : IHC-Fr
Reactivity : Mouse
Masahiro Yasunaga et al. bioRxiv 2018; (Epub) Significant antitumour effect of an antibody against TMEM180, a new cancer-specific molecule.
Application : WB
Reactivity : Human
Xu G et al. Autophagy 2018;:43113 Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1 mRNA splicing.
Application : WB
Reactivity : Human
Sasa K et al. Sci Rep 2018; 8 (1):10579 Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.
Application : ICC/IF
Reactivity : Mouse
Fu JL et al. Hum Reprod 2018; (Epub) Suppression of COUP-TFII upregulates angiogenin and promotes angiogenesis in endometriosis.
Application : WB
Reactivity : Human
Hassan A et al. PLoS One 2018; 13 (5):e0197486 Acute intermittent hypoxia and rehabilitative training following cervical spinal injury alters neuronal hypoxia- and plasticity-associated protein expression.
Application : IHC-P
Reactivity : Rat
Yamamura H et al. Am J Physiol Cell Physiol 2018; (Epub) Hypoxic stress up-regulates Kir2.1 expression by a pathway including hypoxic-inducible factor-1 and dynamin2 in brain capillary endothelial cells.
Ho SY et al. BMC Cancer 2018; 18 (1):480 Development of a computational promoter with highly efficient expression in tumors.
Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Kuang Y et al. Cancer Med 2018; (Epub) Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis.
Application : WB
Reactivity : Human
Owada S et al. Oncol Rep 2018; 39 (4):1805-1812 Autophagy-mediated adaptation of hepatocellular carcinoma cells to hypoxia-mimicking conditions constitutes an attractive therapeutic target.
Kuriyama S et al. Cell Death Discovery 2018; (Epub) PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Application : WB
Reactivity : Human
Lee SH et al. J Tissue Eng Regen Med 2018; (Epub) Improvement of islet function and survival by integration of perfluorodecalin into microcapsules in vivo and in vitro.
Application : ICC/IF
Reactivity : Rat
Lingyan Wang et al. Nat Commun 2017; 8 (0):13876 Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction.
Application : WB
Reactivity : Mouse
Bin? L et al. Oncotarget 2017; 8 (48):83684-83697 Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.
Application : WB
Reactivity : Mouse
Sun W et al. Sci Rep 2017; 7 (1):7190 Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression.
Application : WB
Reactivity : Human
Shih JW et al. Nat Commun 2017; 8:15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Application : WB
Reactivity : Human
Kraus RJ et al. PLoS Pathog 2017; 13 (6):e1006404 Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter.
Application : ICC/IF
Reactivity : Mouse
Wang M et al. Theranostics 2017; 7 (5):1100-1113 AHNAK2 is a Novel Prognostic Marker and Oncogenic Protein for Clear Cell Renal Cell Carcinoma.
Kitajima S et al. Oncotarget 2017; 8 (70):114481-114494 Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells.
Application : WB
Reactivity : Human
Bunai K et al. J Cell Mol Med 2017; (Epub) TMEM207 hinders the tumour suppressor function of WWOX in oral squamous cell carcinoma.
Application : WB
Reactivity : Human
Li S et al. PLoS One 2017; 12 (11):e0187810 Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
Application : WB
Reactivity : Mouse
Ve?e?a1 J et al. Hindawi 2017; (Epub) Neural differentiation is inhibited through HIF1α/β-catenin signaling in embryoid bodies.
Application : WB
Reactivity : Mouse
Zera K et al. PLoS One 2017; 12 (10):e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Owada S et al. Anticancer Res 2017; 37 (9):4927-4934 An Adaptation System to Avoid Apoptosis via Autophagy Under Hypoxic Conditions in Pancreatic Cancer Cells.
Application : WB
Reactivity : Human
Yan Q et al. Cancer Res 2017; (Epub) ANGPTL1 interacts with integrin α1β1 to suppress HCC angiogenesis and metastasis by inhibiting JAK2/STAT3 signaling.
Application : WB
Reactivity : Human
Xu M et al. Front. Pharmacol. 2017; (Epub) E-M, an Engineered Endostatin with High ATPase Activity, Inhibits the Recruitment and Alternative Activation of Macrophages in Non-small Cell Lung Cancer.
Application : WB
Reactivity : Human
Ji Y et al. Nat Commun 2017; 8:15308 Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation.
Application : WB
Reactivity : Human
Ku?era J et al. Hindawi 2017; (Epub) Hypoxia downregulates MAPK/ERK but not STAT3 signaling in ROS-dependent and HIF-1-independent manners in mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
Tsai TH et al. Molecules 2017; 22 (5) Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.
Application : WB
Reactivity : Human
Laubenthal L et al. J Dairy Sci 2017; (Epub) Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows.
Application : IHC-P
Reactivity : Bovine
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Fujiki K et al. J Biol Chem 2017; (Epub) Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Application : WB
Reactivity : Human
Nagamine A et al. JPS 2017; (Epub) The effects of DPP-4 inhibitor on hypoxia-induced apoptosis in human umbilical vein endothelial cells.
Application : ICC/IF
Reactivity : Human
Di K et al. Oncotarget 2016; 7 (47):77457-77467 Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
Application : WB
Reactivity : Human
Jou YC et al. Oncotarget 2016; 7 (40):65403-65417 Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation.
Application : WB
Reactivity : Human
Demetriades C et al. Nat Commun 2016; 7:10662 Lysosomal recruitment of TSC2 is a universal response to cellular stress.
Application : WB
Reactivity : Mouse
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Lin YC et al. Arterioscler Thromb Vasc Biol 2016; (Epub) Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.
Application : WB
Reactivity : Human
Barnes EA et al. FASEB J 2016; (Epub) Loss of smooth muscle cell hypoxia inducible factor-1α underlies increased vascular contractility in pulmonary hypertension.
Application : ICC/IF
Reactivity : Human
Chuang MH et al. Integr Cancer Ther 2016; (Epub) Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.
Application : IHC-P
Reactivity : Human
Wong MJ et al. Am J Physiol Lung Cell Mol Physiol 2016;:ajplung.00345.2016 Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling.
Chiang CH et al. Am J Cancer Res 2016; 6 (8):1785-98 MiR-182 promotes proliferation and invasion and elevates the HIF-1α-VEGF-A axis in breast cancer cells by targeting FBXW7.
Application : WB
Reactivity : Human
Kudova J et al. J Pineal Res 2016; Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization.
Application : WB
Reactivity : Mouse
Lin HC et al. Antioxid Redox Signal 2016; MicroRNA-183 mediates protective postconditioning of the liver by repressing Apaf-1.
Application : WB
Reactivity : Mouse
Logsdon DP et al. Mol Cancer Ther 2016; Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual-Targeting in Patient-Derived 3D Pancreatic Cancer Models.
Application : WB
Reactivity : Human
Lim NS et al. Wound Repair Regen 2016; (Epub) Combination of ciclopirox olamine and sphingosine-1-phosphate as granulation enhancer in diabetic wounds.
Hiromura M et al. Circ J 2016; (Epub) suppressive effects of glucose-dependent *otropic polypeptide on cardiac hypertrophy and fibrosis in angiotensin ii-infused mouse models.
Application : IHC-Fr
Reactivity : Mouse
Tsai SH et al. Sci Rep 2016; 6:28612 Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases.
Application : WB, ChIP assay
Reactivity : Human
Kudova J et al. PLoS One 2016; 11 (6):e0158358 HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
Application : WB
Reactivity : Mouse
Lin CS et al. Int J Mol Sci 2016; 17 (6) Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Application : WB
Reactivity : Human
Erapaneedi R et al. EMBO J 2016; 35 (1):102-13 A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level.
Application : IHC-Fr
Reactivity : Mouse
Jakubauskiene E et al. J Biol Chem 2015; 290 (29):18079-89 Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia.
Application : ChIP assay
Reactivity : Human
Matsuo T et al. Sci Rep 2015; 5:16842 Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.
Application : ICC/IF
Reactivity : Mouse
Kinoshita H et al. Carcinogenesis 2015; 36 (12):1511-20 Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments.
Application : WB
Reactivity : Human
Lucia Bino et al. Chemico-Biological Interactions 2015; The stabilization of hypoxia inducible factor modulates differentiation status and inhibits the proliferation of mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
Stanislava Slade?ek et al. 0 2015; Regulation of mitogen-activated protein kinases phosphatases under hypoxia
Application : WB
Reactivity : Human
Zhu Y et al. Pharmacol Res 2015; 93:36-42 Enhancement of vascular endothelial growth factor release in long-term drug-treated breast cancer via transient receptor potential channel 5-Ca(2+)-hypoxia-inducible factor 1α pathway.
Application : WB
Reactivity : Human
Chen PY et al. Food Funct 2015; 6 (1):286-95 Cytoprotective effects of fisetin against hypoxia-induced cell death in PC12 cells.
Application : WB
Reactivity : Rat
Chakravarthi BV et al. Oncotarget 2014; 5 (16):6654-69 The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.
Application : WB, ChIP assay
Reactivity : Human
Dahlmann M et al. Oncotarget 2014; 5 (10):3220-33 RAGE mediates S100A4-induced cell motility via MAPK/ERK and hypoxia signaling and is a prognostic biomarker for human colorectal cancer metastasis.
Application : WB
Reactivity : Human
Hou HH et al. Respir Res 2014; 15:106 PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.
Application : WB
Reactivity : Human
Morani F et al. J Mol Endocrinol 2014; 53 (2):247-58 PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells.
Pate KT et al. EMBO J 2014; Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.
Application : WB
Reactivity : Human
Chang LC et al. Br J Pharmacol 2014; YC-1 inhibits proliferation of breast cancer cells by downregulating EZH2 expression via activation of c-Cbl and ERK.
Application : WB
Reactivity : Human
Herrera I et al. J Biol Chem 2013; 288 (36):25964-75 Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption.
Application : WB, ICC/IF
Reactivity : Human
Misuno K et al. Stem Cell Res Ther 2013; 4 (6):156 Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
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