GeneTex
United States (US)

HIF1 alpha antibody

Orthogonal
GTX30647_1455_WB_18121410_643.jpg
GTX30647_565_IHC-P_18121410_596.jpg
GTX30647_1456_WB_18121410_201.jpg
GTX30647_334_IHC-P_18121410_292.jpg
GTX30647_221_ICC-IF_18121410_342.jpg

Cat No. GTX30647

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay
Reactivity Human, Mouse, Rat, Rabbit, Goat, Hamster, Monkey, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:100
IHC-P 5 - 10 μg/ml
IHC-Fr 5 - 10 μg/ml
FACS 1:10 - 1:1000
IP 1:10 - 1:500
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

94 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [UniProt# Q61221].

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AA959795 antibody , HIF-1-alpha antibody , HIF1-alpha antibody , HIF1alpha antibody , MOP1 antibody , bHLHe78 antibody

Cellular Localization

Cytoplasm,Nucleus

Background

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.

Database

Research Area

DATA IMAGES
GTX30647_1455_WB_18121410_643.jpg

GTX30647 WB Image

Orthogonal

WB analysis of hypoxia-treated sample using GTX30647 HIF1 alpha antibody.
Lane 1 : CoCl2 treated Cos-7 nuclear extract
Lane 2 : Untreated Cos-7 nuclear extract
Loading : 50μg

GTX30647_565_IHC-P_18121410_596.jpg

GTX30647 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX30647 HIF1 alpha antibody.

GTX30647_1456_WB_18121410_201.jpg

GTX30647 WB Image

WB analysis of (A)PC12 cells, 20% oxygen and (B)PC12 cells, 1% oxygen using GTX30647 HIF1 alpha antibody.

GTX30647_334_IHC-P_18121410_292.jpg

GTX30647 IHC-P Image

IHC-P analysis of human placenta tissue using GTX30647 HIF1 alpha antibody.

GTX30647_221_ICC-IF_18121410_342.jpg

GTX30647 ICC/IF Image

ICC/IF analysis of RCC4 cells using GTX30647 HIF1 alpha antibody.
Green : primary antibody
Blue : Hoechst 33258

REFERENCE