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HIF1 beta antibody [GT1141]

Recombinant antibody
Anti-HIF1 beta antibody [GT1141] used in Western Blot (WB). GTX00799
Anti-HIF1 beta antibody [GT1141] used in IHC (Paraffin sections) (IHC-P). GTX00799
Anti-HIF1 beta antibody [GT1141] used in Western Blot (WB). GTX00799

Cat No. GTX00799

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1141

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan HIF-1 beta

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2

Cellular Localization

Nucleus

Background

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-HIF1 beta antibody [GT1141] used in Western Blot (WB). GTX00799

GTX00799 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 beta antibody [GT1141] (GTX00799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HIF1 beta antibody [GT1141] used in IHC (Paraffin sections) (IHC-P). GTX00799

GTX00799 IHC-P Image

IHC-P analysis of hmuan colon tissue section using GTX00799 HIF1 beta antibody [GT1141].
Dlution : 1:100

Anti-HIF1 beta antibody [GT1141] used in Western Blot (WB). GTX00799

GTX00799 WB Image

WB analysis of HeLa whole cell lysate using GTX00799 HIF1 beta antibody [GT1141].
Dilution : 1:1000
Loading : 25 μg

REFERENCE

There are currently no references for HIF1 beta antibody [GT1141] (GTX00799). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HIF1 beta antibody [GT1141] (GTX00799). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf