GeneTex
United States (US)

HIF1 beta antibody [H1beta234]

GTX30116_1331_WB_18121410_290.jpg
GTX30116_311_IHC-P_18121410_393.jpg
GTX30116_178_ICC-IF_18121410_106.jpg

Cat No. GTX30116

Host Mouse
Clonality Monoclonal
Clone Name H1beta234
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ChIP assay, GSA
Reactivity Human, Mouse, Rat, Sheep, Bovine, Ferret, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:100
IP Assay dependent
ChIP assay 1:10 - 1:500
GSA Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.40 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein containing amino acids 496-789 of human HIF-1 beta. [UniProt# P27540]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aryl Hydrocarbon Receptor Nuclear Translocator,Hif-1-Beta,Hif-1Beta,Hif1-Beta,Hif1B,Hif1Beta,Tango,Bhlhe2,Arnt

Cellular Localization

Nucleus

Background

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30116_1331_WB_18121410_290.jpg

GTX30116 WB Image

WB analysis of HeLa nuclear extract using GTX30116 HIF1 beta antibody [H1beta234].

GTX30116_311_IHC-P_18121410_393.jpg

GTX30116 IHC-P Image

IHC-P analysis of human glioblastoma tissue using GTX30116 HIF1 beta antibody [H1beta234].

GTX30116_178_ICC-IF_18121410_106.jpg

GTX30116 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30116 HIF1 beta antibody [H1beta234].
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 5 μg/ml
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100