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HIF1 beta antibody

Anti-HIF1 beta antibody used in Western Blot (WB). GTX30109
Anti-HIF1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX30109
Anti-HIF1 beta antibody used in Western Blot (WB). GTX30109

Cat No. GTX30109

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay, Gel supershift assays

Reactivity

Human, Mouse, Rat, Sheep, Bovine, Ferret
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF Assay dependent
IHC-P 1:150
IP 1:10 - 1:500
ChIP assay 1:10 - 1:500
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific to HIF1 beta / ARNT. The cross-reactivity of ARNT2 is not determined.

PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2

Cellular Localization

Nucleus

Background

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF1 beta antibody used in Western Blot (WB). GTX30109

GTX30109 WB Image

WB analysis of hypoxic HeLa cell lysate using GTX30109 HIF1 beta antibody.

Anti-HIF1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX30109

GTX30109 IHC-P Image

IHC-P analysis of human skin tissue using GTX30109 HIF1 beta antibody.

Anti-HIF1 beta antibody used in Western Blot (WB). GTX30109

GTX30109 WB Image

WB analysis of MCF-7 cell lysate using GTX30109 HIF1 beta antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 339