GeneTex
United States (US)

HIF1 beta antibody

GTX30109_1328_WB_18121410_676.jpg
GTX30109_309_IHC-P_18121410_278.jpg
GTX30109_1329_WB_18121410_819.jpg

Cat No. GTX30109

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay, GSA
Reactivity Human, Mouse, Rat, Sheep, Bovine, Ferret
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF Assay dependent
IHC-P 1:150
IP 1:10 - 1:500
ChIP assay 1:10 - 1:500
GSA Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific to HIF1 beta / ARNT. The cross-reactivity of ARNT2 is not determined.
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HIF-1-beta antibody , HIF-1beta antibody , HIF1-beta antibody , HIF1B antibody , HIF1BETA antibody , TANGO antibody , bHLHe2 antibody

Cellular Localization

Nucleus

Background

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30109_1328_WB_18121410_676.jpg

GTX30109 WB Image

WB analysis of hypoxic HeLa cell lysate using GTX30109 HIF1 beta antibody.

GTX30109_309_IHC-P_18121410_278.jpg

GTX30109 IHC-P Image

IHC-P analysis of human skin tissue using GTX30109 HIF1 beta antibody.

GTX30109_1329_WB_18121410_819.jpg

GTX30109 WB Image

WB analysis of MCF-7 cell lysate using GTX30109 HIF1 beta antibody.