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HIF1AN antibody

Anti-HIF1AN antibody used in Immunohistochemistry (IHC). GTX30646
Anti-HIF1AN antibody used in Western Blot (WB). GTX30646

Cat. No. GTX30646

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, EM, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($429)
See all HIF1AN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
IHC-Fr 1:50 - 1:200
IP 1:10 - 1:500
EM Assay dependent
IHC 1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha inhibitor , FIH1

Cellular Localization

Nucleus,Cytoplasm

Background

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

Database

Research Area

DATA IMAGES

Anti-HIF1AN antibody used in Immunohistochemistry (IHC). GTX30646

GTX30646 IHC Image

IHC analysis of rat brain tissue using GTX30646 HIF1AN antibody.

Anti-HIF1AN antibody used in Western Blot (WB). GTX30646

GTX30646 WB Image

WB analysis of astrocyte lysate from rat brain using GTX30646 HIF1AN antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 429