GeneTex
United States (US)

HIF1AN antibody

GTX30646_333_IHC_18121410_475.jpg
GTX30646_1454_WB_18121410_352.jpg

Cat No. GTX30646

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, EM, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:5000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
IHC-Fr 1:50 - 1:200
IP 1:10 - 1:500
EM Assay dependent
IHC 1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor,Fih1,Hif1An

Cellular Localization

Nucleus

Background

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

Database

Research Area

DATA IMAGES
GTX30646_333_IHC_18121410_475.jpg

GTX30646 IHC Image

IHC analysis of rat brain tissue using GTX30646 HIF1AN antibody.

GTX30646_1454_WB_18121410_352.jpg

GTX30646 WB Image

WB analysis of astrocyte lysate from rat brain using GTX30646 HIF1AN antibody.
Dilution : 1:500

Package List Price ($)
$ 339