GeneTex
  • Country / Location Selection

United States (US)

HIF1AN antibody

Anti-HIF1AN antibody used in Immunohistochemistry (IHC). GTX30646
Anti-HIF1AN antibody used in Western Blot (WB). GTX30646

Cat No. GTX30646

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, EM, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:5000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
IHC-Fr 1:50 - 1:200
IP 1:10 - 1:500
EM Assay dependent
IHC 1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha inhibitor , FIH1

Cellular Localization

Nucleus,Cytoplasm

Background

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.

Database

Research Area

DATA IMAGES

Anti-HIF1AN antibody used in Immunohistochemistry (IHC). GTX30646

GTX30646 IHC Image

IHC analysis of rat brain tissue using GTX30646 HIF1AN antibody.

Anti-HIF1AN antibody used in Western Blot (WB). GTX30646

GTX30646 WB Image

WB analysis of astrocyte lysate from rat brain using GTX30646 HIF1AN antibody.
Dilution : 1:500

REFERENCE

There are currently no references for HIF1AN antibody (GTX30646). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for HIF1AN antibody (GTX30646). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339