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HIF1AN antibody

Anti-HIF1AN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32652
Anti-HIF1AN antibody used in Immunoprecipitation (IP). GTX32652
Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX32652
Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX32652
Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX32652
Anti-HIF1AN antibody used in Western Blot (WB). GTX32652

Cat No. GTX32652

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329)
See all HIF1AN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN (NP_060372.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha inhibitor , FIH1

Cellular Localization

Nucleus,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-HIF1AN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32652

GTX32652 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32652 HIF1AN antibody.
Blue : DAPI
Dilution : 1:100

Anti-HIF1AN antibody used in Immunoprecipitation (IP). GTX32652

GTX32652 IP Image

IP analysis of 293T cells using GTX32652 HIF1AN antibody.
IP reaction : 200μg extracts / 1μg antibody antibody
Dilution : 1:1000

Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX32652

GTX32652 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32652 HIF1AN antibody.
Dilution : 1:100

Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX32652

GTX32652 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX32652 HIF1AN antibody.
Dilution : 1:100

Anti-HIF1AN antibody used in IHC (Paraffin sections) (IHC-P). GTX32652

GTX32652 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX32652 HIF1AN antibody.
Dilution : 1:100

Anti-HIF1AN antibody used in Western Blot (WB). GTX32652

GTX32652 WB Image

WB analysis of various sample lysates using GTX32652 HIF1AN antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329