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United States (US)

HIF2 alpha antibody [GT125]

GTX632015_42023_20150924_IFA_18121411_378.jpg
GTX632015_42023_20150924_IFA_2_18121411_300.jpg
GTX632015_42023_20151030_IFA_18121411_762.jpg
GTX632015_42023_20151016_IP_18121411_518.jpg
GTX632015_42023_20150313_WB_Hypoxia_18121411_951.jpg

Cat No. GTX632015

Host Mouse
Clonality Monoclonal
Clone Name GT125
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:700
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HepG2 (1% O2 treatment for 24hr)

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Endothelial Pas Domain Protein 1,Ecyt4,Hif2A,Hlf,Mop2,Pasd2,Bhlhe73,Epas1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES
GTX632015_42023_20150924_IFA_18121411_378.jpg

GTX632015 ICC/IF Image

HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Red: Actin filaments were labeled with Phalloidin.

GTX632015_42023_20150924_IFA_2_18121411_300.jpg

GTX632015 ICC/IF Image

HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX632015_42023_20151030_IFA_18121411_762.jpg

GTX632015 ICC/IF Image

HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Samples: PC-12 cells mock (left) and treated with 500 μM CoCl2 for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Blue: Hoechst 33342 staining.

GTX632015_42023_20151016_IP_18121411_518.jpg

GTX632015 IP Image

Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody [GT125] (GTX632015).
Western blot analysis was performed using HIF2 alpha antibody [GT125] (GTX632015).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX632015_42023_20150313_WB_Hypoxia_18121411_951.jpg

GTX632015 WB Image

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX632015) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

HIF2 alpha antibody [GT125] Cat No. GTX632015

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA, 2Hr
Permeabilization /Antigen retrieval : Citrate buffer, 100°C, 30minHr
Primary Antibody : 1:400, 25°C, 0.5Hr
SDS
PBS.pdf