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HIF2 alpha antibody [N1N3]

Anti-HIF2 alpha antibody [N1N3] used in Western Blot (WB). GTX103707

Cat No. GTX103707

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ChIP assay

Reactivity

Human, Zebrafish
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HepG2 (1% O2 treatment for 24hr) , *HT1080 , *786-0 , *FADU

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617613

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endothelial PAS domain protein 1 , ECYT4 , HIF2A , HLF , MOP2 , PASD2 , bHLHe73

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HIF2 alpha antibody [N1N3] used in Western Blot (WB). GTX103707

GTX103707 WB Image

HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract (1% O2 treatment for 24 hr)
7.5% SDS-PAGE
HIF2 alpha antibody (GTX103707) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

HIF2 alpha antibody [N1N3] Cat No. GTX103707

Rating ( Average 4.6 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 4 users reviews)
ChIP assay(ChIP assay) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25000307
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncol Lett in 2015.PMID: 26622817
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncol Lett in 2015.PMID: 26622817
Date : Anonymous submitted on 25-Nov-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT1080,786-0 cells
Amount used : 50μg
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A: HT1080 cells,B: 786-0 cells
Figure Note : Dr Norazizah Shafee, Universiti Putra Malaysia
Date : Anonymous submitted on 22-Dec-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : FADU cells
Amount used : 40μg
Blocking : 4% BSA, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf